Abstract
The synthesis of carrier-free β-32P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free 32P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-32P-nucleoside diphosphate.
Original language | English |
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Pages (from-to) | 198-202 |
Number of pages | 5 |
Journal | Analytical Biochemistry |
Volume | 109 |
Issue number | 1 |
DOIs | |
State | Published - 1980 |
Externally published | Yes |