Synthesis of carrier-free β-32P-nucleosides-triphosphate in almost quantitative yields

Gabriel Kaufmann, Mordechai Choder, Yoram Groner

Research output: Contribution to journalArticlepeer-review

Abstract

The synthesis of carrier-free β-32P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free 32P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-32P-nucleoside diphosphate.

Original languageEnglish
Pages (from-to)198-202
Number of pages5
JournalAnalytical Biochemistry
Volume109
Issue number1
DOIs
StatePublished - 1980
Externally publishedYes

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