Synthesis of carrier-free β-32P-nucleosides-triphosphate in almost quantitative yields

Gabriel Kaufmann; Mordechai Choder; Yoram Groner

doi:10.1016/0003-2697(80)90029-9

Synthesis of carrier-free β-³²P-nucleosides-triphosphate in almost quantitative yields

Gabriel Kaufmann^*, Mordechai Choder, Yoram Groner

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The synthesis of carrier-free β-³²P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free ³²P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-³²P-nucleoside diphosphate.

Original language	English
Pages (from-to)	198-202
Number of pages	5
Journal	Analytical Biochemistry
Volume	109
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(80)90029-9
State	Published - 1980
Externally published	Yes

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10.1016/0003-2697(80)90029-9

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@article{968a3c26b89249a99f787304335ba8ac,

title = "Synthesis of carrier-free β-32P-nucleosides-triphosphate in almost quantitative yields",

abstract = "The synthesis of carrier-free β-32P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free 32P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-32P-nucleoside diphosphate.",

author = "Gabriel Kaufmann and Mordechai Choder and Yoram Groner",

note = "Funding Information: I This work was supported by U S-Israel Science Foundation Grants 1315 to Y.G. G.K: and by a grant from the Israel National of Science to G.K. {\textquoteright} Incumbent of the Alan Dixon Career ment Chair. ” Incumbent Development",

year = "1980",

doi = "10.1016/0003-2697(80)90029-9",

language = "אנגלית",

volume = "109",

pages = "198--202",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - Synthesis of carrier-free β-32P-nucleosides-triphosphate in almost quantitative yields

AU - Kaufmann, Gabriel

AU - Choder, Mordechai

AU - Groner, Yoram

N1 - Funding Information: I This work was supported by U S-Israel Science Foundation Grants 1315 to Y.G. G.K: and by a grant from the Israel National of Science to G.K. ’ Incumbent of the Alan Dixon Career ment Chair. ” Incumbent Development

PY - 1980

Y1 - 1980

N2 - The synthesis of carrier-free β-32P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free 32P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-32P-nucleoside diphosphate.

AB - The synthesis of carrier-free β-32P-ribonucleosides triphosphate in almost quantitative yields is described. The procedures are based on a polynucleotide phosphorylase-catalyzed phosphorolysis of either poly- or oligoribonucleotides with carrier-free 32P-inorganic phosphate, coupled with a pyruvate kinase-catalyzed phosphorylation of the resulting β-32P-nucleoside diphosphate.

UR - http://www.scopus.com/inward/record.url?scp=0019326240&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(80)90029-9

DO - 10.1016/0003-2697(80)90029-9

M3 - מאמר

AN - SCOPUS:0019326240

VL - 109

SP - 198

EP - 202

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -

Synthesis of carrier-free β-³²P-nucleosides-triphosphate in almost quantitative yields

Abstract

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