Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates

Dragoslav Zikich; Natalia Borovok; Tatiana Molotsky; Alexander Kotlyar

doi:10.1021/bc900527a

Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates

Dragoslav Zikich, Natalia Borovok, Tatiana Molotsky, Alexander Kotlyar^*

^*Corresponding author for this work

Biochemistry Molecular Biology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiolfunctionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo^- polymerase in the presence of dCTP and dGTP.

Original language	English
Pages (from-to)	544-547
Number of pages	4
Journal	Bioconjugate Chemistry
Volume	21
Issue number	3
DOIs	https://doi.org/10.1021/bc900527a
State	Published - 17 Mar 2010

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abstract = "In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiolfunctionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo- polymerase in the presence of dCTP and dGTP.",

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AU - Molotsky, Tatiana

AU - Kotlyar, Alexander

PY - 2010/3/17

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N2 - In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiolfunctionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo- polymerase in the presence of dCTP and dGTP.

AB - In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiolfunctionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo- polymerase in the presence of dCTP and dGTP.

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Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates

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