Synthesis and AFM characterization of poly(dG)-poly(dC)-gold nanoparticle conjugates

Dragoslav Zikich, Natalia Borovok, Tatiana Molotsky, Alexander Kotlyar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In the present work, we have synthesized conjugates between the 5 nm gold nanoparticles (Au-NP) and 5' thiolfunctionalized, 700 bp poly(dG)-poly(dC). We have completely separated and purified to homogeneity conjugates bearing different number of poly(dG)-poly(dC) molecules per Au-NP by electrophoresis and HPLC. The conjugates were directly visualized by atomic force microscopy. We have demonstrated that Au NP-bound poly(dG)-poly(dC) can be considerably extended by Klenow exo- polymerase in the presence of dCTP and dGTP.

Original languageEnglish
Pages (from-to)544-547
Number of pages4
JournalBioconjugate Chemistry
Volume21
Issue number3
DOIs
StatePublished - 17 Mar 2010

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