Synovial VLA-1+ T cells display an oligoclonal and partly distinct repertoire in rheumatoid and psoriatic arthritis

Itamar Goldstein*, Amos J. Simon, Shomron Ben Horin, Sarit Matzri, Alexander Koltakov, Pnina Langevitz, Gideon Rechavi, Ninette Amariglio, Ilan Bank

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

VLA-1 integrin expressing T cells are more frequent in inflammatory synovial fluids (SF) compared to peripheral blood. Recent studies suggest that VLA-1 expression mainly marks IFNγ+ T cells while excluding both IL-4+ and regulatory FoxP3+ T cells. To further characterize the TCR repertoire of the potentially pathogenic VLA-1+ IFNγ+ T cells, isolated from SF of adult patients with rheumatoid and psoriatic arthritis, we determined the complementarity determining region (CDR)3 spectratypes. Here we show in a cohort of 9 patients that VLA-1+ T cells display a perturbed repertoire that, moreover, differs from that of VLA-1- synovial T cells and even VLA-1+ PB T cells. Importantly, random sequencing of the CDR3 region of the TCR variable β (BV) 6.1 gene of both VLA-1+ and VLA-1- synovial T cells, in one patient, revealed that their sequences were by and large different (29 out of 33 clones). Thus, our results imply that VLA-1+ T cells that infiltrate into inflamed joints represent a partly distinct and highly oligoclonal population of Th1 cells, probably selected by unique antigens.

Original languageEnglish
Pages (from-to)75-84
Number of pages10
JournalClinical Immunology
Volume128
Issue number1
DOIs
StatePublished - Jul 2008

Funding

FundersFunder number
Porter School of Environmental Studies, Tel Aviv University

    Keywords

    • CDR3 length diversity
    • Effector and memory T cells
    • Inflammatory arthritis
    • Integrin
    • T cell receptor (TCR)
    • TCR repertoire
    • Very late activation antigen (VLA-1)

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