Synergistic effect of heat and solar UV on DNA damage and water disinfection of E. Coli and bacteriophage MS2

Dana Jennifer Theitler, Abid Nasser, Yoram Gerchman, Abraham Kribus, Hadas Mamane*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The response of a representative virus and indicator bacteria to heating, solar irradiation, or their combination, was investigated in a controlled solar simulator and under real sun conditions. Heating showed higher inactivation of Escherichia coli compared to the bacteriophage MS2. Heating combined with natural or simulated solar irradiation demonstrated a synergistic effect on the inactivation of E. coli, with up to 3-log difference for 50 °C and natural sun insolation of 2,000 kJ m-2 (compared to the sum of the separate treatments). Similar synergistic effect was also evident when solar-UV induced DNA damage to E. coli was assessed using the endonuclease sensitive site assay (ESS). MS2 was found to be highly resistant to irradiation and heat, with a slightly synergistic effect observed only at 59 WC and natural sun insolation of 5,580 kJ m-2. Heat treatment also hindered lightdependent recovery of E. coli making the treatment much more effective.

Original languageEnglish
Pages (from-to)605-618
Number of pages14
JournalJournal of Water and Health
Volume10
Issue number4
DOIs
StatePublished - 2012

Keywords

  • DNA damage
  • Endonuclease sensitive site (ESS)
  • Heat inactivation
  • Insolation
  • Recovery
  • Solar disinfection

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