Synergism between aflatoxins in covalent binding to DNA and in mutagenesis in the photoactivation system

Avishay Abraham Stark*, Daniel F. Liberman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Aflatoxins (AFs) produce singlet oxygen upon their exposure to UV (365-nm) light. Singlet oxygen in turn activates them to mutagens and DNA-binding species. DNA binding and mutagenesis by AFs were enhanced in D2O as compared to reactions in H2O, and a singlet oxygen scavenger inhibited mutagenesis. DNA photobinding of 3H-AFB1 increased in the presence of unlabeled AFB2, and the addition of AFB2 enhanced mutagenesis by AFB1 in a synergistic manner. These results are compatible with the notion that singlet oxygen, formed by one aflatoxin molecule, can readily activate another aflation molecule. This may bear an environmental implication in that the weakly carcinogenic AFB2, which is often produced in nature together with AFB1, may be important in enhancing the activation of AFB1 by sunlight.

Original languageEnglish
Pages (from-to)77-86
Number of pages10
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume247
Issue number1
DOIs
StatePublished - Mar 1991

Keywords

  • Aflatoxins
  • DNA binding
  • Mutagenesis
  • Photoactivation
  • Singlet oxygen

Fingerprint

Dive into the research topics of 'Synergism between aflatoxins in covalent binding to DNA and in mutagenesis in the photoactivation system'. Together they form a unique fingerprint.

Cite this