Synaptotagmin II negatively regulates MHC class II presentation by mast cells

Dana Baram, Ze Peng, Ora Medalia, Yoseph A. Mekori, Ronit Sagi-Eisenberg

Research output: Contribution to journalArticlepeer-review

Abstract

Synaptotagmins (Syts), comprise a gene family of proteins, implicated in the control of protein traffic. Rat basophilic leukemia cells (RBL-2H3), a tumor analogue of mucosal mast cells (MMC), express at least four distinct Syt homologues, including Syt II, Syt III, Syt V and Syt IX. Synaptotagmin II is located at the late/endosomal/lysosomal compartment, where it negatively regulates lysosomal exocytosis. Mast cells may contribute to immune defense mechanisms by presenting MHC class II/antigen complexes and triggering T cell-dependent immune responses. We now demonstrate that RBL-2H3 mast cells, which express reduced levels of Syt II (<5%) by transfection with Syt II antisense cDNA, are able to release MHC class II molecules. We further show that release of both MHC class II molecules and of the lysosomal enzyme cathepsin D is stimulated by lipopolysaccharide (LPS, 1μg/ml, 48h). We show further that LPS reduces by >40% the level of Syt II expression in both RBL-2H3 and bone marrow-derived mast cells (BMMC). This effect is both dose and time-dependent. These results indicate that Syt II can be down-regulated by external inflammatory signals, resulting in the amplification of mast cell function. Finally, our results implicate Syt II as an important and novel regulator of MHC class II presentation.

Original languageEnglish
Pages (from-to)1347-1352
Number of pages6
JournalMolecular Immunology
Volume38
Issue number16-18
DOIs
StatePublished - 2002

Keywords

  • IgE
  • MHC class II
  • Mast cells
  • RBL-2H3
  • Synaptotagmin

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