TY - JOUR
T1 - Susceptibility of human cumulus cells to bisphenol a In vitro
AU - Mansur, Abdallah
AU - Adir, Michal
AU - Racowsky, Catherine
AU - Combelles, Catherine MH
AU - Landa, Natalie
AU - Machtinger, Ronit
N1 - Publisher Copyright:
© 2017 Elsevier Inc.
PY - 2017/12
Y1 - 2017/12
N2 - Bisphenol A (BPA) is detectable in follicular fluid. However, the effect of BPA exposure on human cumulus cells (CC) that surround the oocyte and are crucial for oocyte competence has been largely unexplored. We exposed primary cultures of CC to increasing concentrations of BPA [0,0.002, 0.02 and 20 μg/mL] and tested the effects of BPA on the expression of genes associated with apoptosis using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR); we also assessed the effect of BPA on apoptosis by staining with anti-caspase 3. Exposure to 20 μg/mL BPA led to significantly decreased expression of CDC20, BUB1 B and HAS2 (p < 0.03), increased expression of TRIB3 and LUM (p ≤ 0.005), and increased frequency of cells positive for anti-CASP3 (p = 0.03), compared to control. Our results imply that BPA may lead to ovarian toxicity by increasing CC apoptosis and provide an important molecular mechanism for the effect of BPA on human CC in vitro.
AB - Bisphenol A (BPA) is detectable in follicular fluid. However, the effect of BPA exposure on human cumulus cells (CC) that surround the oocyte and are crucial for oocyte competence has been largely unexplored. We exposed primary cultures of CC to increasing concentrations of BPA [0,0.002, 0.02 and 20 μg/mL] and tested the effects of BPA on the expression of genes associated with apoptosis using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR); we also assessed the effect of BPA on apoptosis by staining with anti-caspase 3. Exposure to 20 μg/mL BPA led to significantly decreased expression of CDC20, BUB1 B and HAS2 (p < 0.03), increased expression of TRIB3 and LUM (p ≤ 0.005), and increased frequency of cells positive for anti-CASP3 (p = 0.03), compared to control. Our results imply that BPA may lead to ovarian toxicity by increasing CC apoptosis and provide an important molecular mechanism for the effect of BPA on human CC in vitro.
KW - Apoptosis
KW - Bisphenol A
KW - Cumulus cells
KW - Human primary cultures
UR - http://www.scopus.com/inward/record.url?scp=85030783683&partnerID=8YFLogxK
U2 - 10.1016/j.reprotox.2017.09.008
DO - 10.1016/j.reprotox.2017.09.008
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C2 - 28951153
AN - SCOPUS:85030783683
SN - 0890-6238
VL - 74
SP - 189
EP - 194
JO - Reproductive Toxicology
JF - Reproductive Toxicology
ER -
