TY - JOUR
T1 - Surfactant protein d-coated klebsiella pneumoniae stimulatescytokine production in mononuclear phagocytes
AU - Keisari, Yona
AU - Wang, Hongbin
AU - Mesika, Adi
AU - Matatov, Robert
AU - Nissimov, Liat
AU - Crouch, Erika
AU - Ofek, Itzhak
PY - 2001
Y1 - 2001
N2 - Encapsulated Klebsiella pneumoniae strains K21a, K10, and K50, all of which contain dimannose sequences in their capsular polysaccharides that are recognized by the mannose receptor of macrophages, stimulated interleukin secretion and cytokine mRNA expression by human monocyte-derived macrophages. By contrast, the corresponding unencapsulated phase variants and the K2 strain, which lack the dimannose sequence, did not. Coating of unencapsulated phase variants of Klebsiella strains with surfactant protein (SP)-D resulted in marked stimulation of cytokine mRNA accumulation. The induction of cytokine mRNA via the mannose receptor occurred only in monocyte-derived macrophages, whereas that caused by SP-D-coated Klebsiella strains occurred in both macrophages and peripheral-blood monocytes. The results suggested that innate immunity against pulmonary pathogens might be mediated by SP-D, which acts as an opsonin to enhance the interaction of macrophages with unencapsulated phase variants originating from the upper respiratory tract, and by macrophage mannose receptors, which recognize encapsulated variants expressing capsular dimannose residues.
AB - Encapsulated Klebsiella pneumoniae strains K21a, K10, and K50, all of which contain dimannose sequences in their capsular polysaccharides that are recognized by the mannose receptor of macrophages, stimulated interleukin secretion and cytokine mRNA expression by human monocyte-derived macrophages. By contrast, the corresponding unencapsulated phase variants and the K2 strain, which lack the dimannose sequence, did not. Coating of unencapsulated phase variants of Klebsiella strains with surfactant protein (SP)-D resulted in marked stimulation of cytokine mRNA accumulation. The induction of cytokine mRNA via the mannose receptor occurred only in monocyte-derived macrophages, whereas that caused by SP-D-coated Klebsiella strains occurred in both macrophages and peripheral-blood monocytes. The results suggested that innate immunity against pulmonary pathogens might be mediated by SP-D, which acts as an opsonin to enhance the interaction of macrophages with unencapsulated phase variants originating from the upper respiratory tract, and by macrophage mannose receptors, which recognize encapsulated variants expressing capsular dimannose residues.
KW - Capsular polysaccharides
KW - Capsule
KW - Human
KW - Macrophages
KW - Mannose receptor
KW - Monocytes
UR - http://www.scopus.com/inward/record.url?scp=0034964376&partnerID=8YFLogxK
U2 - 10.1189/jlb.70.1.135
DO - 10.1189/jlb.70.1.135
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0034964376
SN - 0741-5400
VL - 70
SP - 135
EP - 141
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 1
ER -