Suppression of myosin-induced and adoptively transferred myocarditis by prior treatment with complete Freund's adjuvant

Jacob George, Arnon Adler, Iris Barshack, Gad Keren, Arie Roth

Research output: Contribution to journalArticlepeer-review

Abstract

Background We evaluated the effect of pretreatment with complete Freund's adjuvant (CFA) on experimentally induced myocarditis concomitant with the assessment of antigen-specific properties of disease-triggering lymphocytes. Methods and results Rats pretreated with CFA a week prior to myosin immunization developed a significantly attenuated myocardial inflammation as compared to control-treated animals. Furthermore, prior administration of CFA virtually abolished histological evidence of myocarditis induced by transfer of antimyosin lymphocytes. CFA administered subcutaneously prior to myosin immunization resulted in a significant reduction in lymph node cell reactivity to myosin. Assessment of cultured medium from lymphocytes obtained from CFA-pretreated myosin-immunized rats revealed reduced levels of interferon gamma but an increased production of IL-10, suggesting an induction of a Thl to Th2 switch. Conclusions Thus, CFA treatment suppressed both myosin-induced as well as adoptively transferred myocarditis concomitant with induction of antigen-specific unresponsiveness to myosin and skewing of the immune response in favor of a Th2-dominated profile.

Original languageEnglish
Pages (from-to)221-224
Number of pages4
JournalCardiovascular Pathology
Volume13
Issue number4
DOIs
StatePublished - Jul 2004
Externally publishedYes

Keywords

  • Adjuvant
  • Immune system
  • Myocarditis
  • Myosin
  • Rat

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