Suppression of experimental autoimmune encephalomyelitis by intravenously administered polyclonal immunoglobulins

A. Achiron, F. Mor, R. Margalit, I. R. Cohen, O. Lider, S. Miron

Research output: Contribution to journalArticlepeer-review

Abstract

Experimental autoimmune encephalomyelitis (EAE) was induced in Lewis rats either by active immunization with myelin basic protein (MBP) or by adoptive transfer using anti-MBP specific CD4+ T cells. Treatment with human polyclonal immunoglobulins (IgG) effectively suppressed active EAE. Time-dependent experiments demonstrated that the effect of IgG was manifested only when treatment was given immediately after immunization; administration from day 7 after disease induction did not suppress the disease. In the adoptive transfer model of EAE, IgG had no effect in vivo. However, pretreatment in vitro of the antigen-specific T-cells with IgG inhibited their ability to mediate adoptive EAE, as it did in active EAE. Similarly, in vitro IgG pretreatment of the antigen-specific T-cells suppressed the proliferative response to MBP. Fluorescent Activated Cell Sorter (FACS) analysis demonstrated the binding of IgG to activated T-cell lines that was inhibited by soluble Fc molecules. The differential effects of IgG on active EAE and on the adoptive transfer of EAE suggest that IgG in vivo can suppress disease by acting during the early phase of the immune response which involves naive T cells. The inhibition of T-cell proliferation and adoptive transfer of EAE by incubation of T cells in vitro appears to require higher concentrations of IgG than those obtained in vivo. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)323-330
Number of pages8
JournalJournal of Autoimmunity
Volume15
Issue number3
DOIs
StatePublished - 2000
Externally publishedYes

Keywords

  • Autoimmunity
  • Encephalitogenic T-cells
  • Experimental autoimmune encephalomyelitis
  • Immunoglobulins

Fingerprint

Dive into the research topics of 'Suppression of experimental autoimmune encephalomyelitis by intravenously administered polyclonal immunoglobulins'. Together they form a unique fingerprint.

Cite this