Superoxide anion and hydrogen peroxide production by chemically elicited peritoneal macrophages-Induction by multiple nonphagocytic stimuli

Edgar Pick*, Yona Keisari

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The ability of a number of stimulants to activate an oxidative burst (OB) in oil-elicited guinea pig peritoneal exudate macrophages (MPs) was examined. The parameters of the OB were the generation and extracellular release of Superoxide anions (O2-) and hydrogen peroxide (H2O2). We found that: (1) The cocarcinogen and skin irritant phorbol myristate acetate (PMA) was the most potent OB activator-The weak cocarcinogen 4-O-methyl PMA was a proportionally less effective OB activator; (2) The lectins concanavalin A (Con A) and wheat germ agglutinin (WGA), but not soybean, Lotus, and pokeweed lectins, were also quite effective OB activators-The ability of Con A to stimulate O2- production was abolished by succinylation and could be prevented by the presence of α-methyl-D-mannoside; (3) Other stimulators of an OB in MPs were: N-formyl-methionyl peptides, opsonized zymosan, the Ca2+ ionophore A23187, phospholipase C, NaF, antimacrophage antibody, microtubule-disrupting drugs, and sodium nitroprusside-O2- generation induced by A23187 (but not that stimulated by PMA) was dependent on extracellular Ca2+; (4) The amount of O2- produced per cell was higher at low cell densities; (5) The addition of Superoxide dismutase (SOD) to the medium totally prevented the detection of O2- and augmented twice the amount of H2O2 recovered; (6) Pretreatment of MPs with the SOD inhibitor sodium diethyldithiocarbamate had no effect on the release of O2- but blocked H2O2 release in a dose-dependent manner. These data were interpreted as indicating that the bulk of H2O2 was derived by enzymatic dismutation of O2-; (7) The common mechanism by which such a variety of stimuli provoke an OB in MPs was not elucidated. No evidence was found to suggest a role for a cyclic nucleotide messenger.

Original languageEnglish
Pages (from-to)301-318
Number of pages18
JournalCellular Immunology
Volume59
Issue number2
DOIs
StatePublished - Apr 1981

Funding

FundersFunder number
German Cancer Research Center1505
National Council for Forest Research and Development
United States-Israel Binational Science Foundation

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