Super-SILAC: Current trends and future perspectives

Anjana Shenoy, Tamar Geiger*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Stable isotope labeling with amino acids in cell culture (SILAC) has risen as a powerful quantification technique in mass spectrometry (MS)-based proteomics in classical and modified forms. Previously, SILAC was limited to cultured cells because of the requirement of active protein synthesis; however, in recent years, it was expanded to model organisms and tissue samples. Specifically, the super-SILAC technique uses a mixture of SILAC-labeled cells as a spike-in standard for accurate quantification of unlabeled samples, thereby enabling quantification of human tissue samples. Here, we highlight the recent developments in super-SILAC and its application to the study of clinical samples, secretomes, post-translational modifications and organelle proteomes. Finally, we propose super-SILAC as a robust and accurate method that can be commercialized and applied to basic and clinical research.

Original languageEnglish
Pages (from-to)13-19
Number of pages7
JournalExpert Review of Proteomics
Volume12
Issue number1
DOIs
StatePublished - 1 Feb 2014

Funding

FundersFunder number
Gene Regulation in Complex Human Disease Center41/11
Israel Science Foundation-Israeli Centers of Research Excellence
Israel Cancer Research Fund
Israeli Centers for Research Excellence

    Keywords

    • biomarkers
    • cancer
    • clinical proteomics
    • mass spectrometry
    • quantitative proteomics
    • spike-in standard
    • stable isotope labeling
    • super-SILAC

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