[3H]GBR 12935 labels mainly the piperazine acceptor site in the rat prefrontal cortex

Irit Gordon, Ronit Weizman, Moshe Rehavi*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


The binding characteristics of [3H]GBR 12935, a ligand for the dopamine (DA) transporter, have been extensively investigated in the striatum. The present study was designed to characterize L3H]GBR 12935-binding to prefrontal cortex (PFC) in rats. This region receives a dense DA input from the ventral tegmental area and is suspected to play a major role in higher associative functions. We demonstrated high-affinity, saturable, mazindol-sensitive [3H]GBR 12935-binding in the rat PFC; however, in contrast to the striatum, such binding was inhibited by increasing concentrations of Na+. This fact, together with the irregular pattern of the association kinetics and the marked sensitivity of [3H]GBR 12935-binding to piperazine derivatives, indicates the possible presence of more than one [3H]GBR 12935-binding site in the PFC. Furthermore, it appears that [3H]GBR 12935 in the rat PFC labels mainly 'the piperazine acceptor site' and not the DA transporter.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalBrain Research
Issue number2
StatePublished - 20 Mar 1995


FundersFunder number
Offer Foundation for Psychiatric Research
Recanati Foundation
Sackler Faculty of Medicine
Tel-Aviv University
Office of the Chief Scientist, Ministry of Health


    • Dopamine transporter
    • Dopamine uptake site
    • Piperazine acceptor site
    • Prefrontal cortex
    • Striatum
    • [H]GBR 12935


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