TY - JOUR
T1 - [3H]GBR 12935 labels mainly the piperazine acceptor site in the rat prefrontal cortex
AU - Gordon, Irit
AU - Weizman, Ronit
AU - Rehavi, Moshe
N1 - Funding Information:
This research was supported by the Offer Foundation for Psychiatric Research, the Recanati Foundation (Tel-Aviv University, Sackler Faculty of Medicine) and the Chief Scientist of the Israeli Ministry of Health.
PY - 1995/3/20
Y1 - 1995/3/20
N2 - The binding characteristics of [3H]GBR 12935, a ligand for the dopamine (DA) transporter, have been extensively investigated in the striatum. The present study was designed to characterize L3H]GBR 12935-binding to prefrontal cortex (PFC) in rats. This region receives a dense DA input from the ventral tegmental area and is suspected to play a major role in higher associative functions. We demonstrated high-affinity, saturable, mazindol-sensitive [3H]GBR 12935-binding in the rat PFC; however, in contrast to the striatum, such binding was inhibited by increasing concentrations of Na+. This fact, together with the irregular pattern of the association kinetics and the marked sensitivity of [3H]GBR 12935-binding to piperazine derivatives, indicates the possible presence of more than one [3H]GBR 12935-binding site in the PFC. Furthermore, it appears that [3H]GBR 12935 in the rat PFC labels mainly 'the piperazine acceptor site' and not the DA transporter.
AB - The binding characteristics of [3H]GBR 12935, a ligand for the dopamine (DA) transporter, have been extensively investigated in the striatum. The present study was designed to characterize L3H]GBR 12935-binding to prefrontal cortex (PFC) in rats. This region receives a dense DA input from the ventral tegmental area and is suspected to play a major role in higher associative functions. We demonstrated high-affinity, saturable, mazindol-sensitive [3H]GBR 12935-binding in the rat PFC; however, in contrast to the striatum, such binding was inhibited by increasing concentrations of Na+. This fact, together with the irregular pattern of the association kinetics and the marked sensitivity of [3H]GBR 12935-binding to piperazine derivatives, indicates the possible presence of more than one [3H]GBR 12935-binding site in the PFC. Furthermore, it appears that [3H]GBR 12935 in the rat PFC labels mainly 'the piperazine acceptor site' and not the DA transporter.
KW - Dopamine transporter
KW - Dopamine uptake site
KW - Piperazine acceptor site
KW - Prefrontal cortex
KW - Striatum
KW - [H]GBR 12935
UR - http://www.scopus.com/inward/record.url?scp=0028986489&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(95)00007-D
DO - 10.1016/0006-8993(95)00007-D
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AN - SCOPUS:0028986489
SN - 0006-8993
VL - 674
SP - 205
EP - 210
JO - Brain Research
JF - Brain Research
IS - 2
ER -