TY - JOUR
T1 - 32P incorporation and growth of the hydrocarbon-degrading pseudomonad UP-2
AU - Kirschner Zillber, Ilana
AU - Gutnick, David
AU - Rosenberg, Eugene
PY - 1979/5
Y1 - 1979/5
N2 - The marine pseudomonad UP-2 grew, with a doubling time of 1.5 h at 30°C, in media supplemented with n-tetracosane as the sole organic carbon and energy source. Over 99% of the solid hydrocarbon was degraded. In order to overcome serious problems of sampling and measuring growth in the biphase system of hydrocarbon-water, a technique was developed combining the use of separate 1-ml eultures for each sample and32P labeling as an assay for growth. Using this technique, a growth durve of UP-2 on n-tetracosane was obtained. UP-2 pregrown on sodium acetate can utilize even-chain, liquid, or solid n-alkanes with no observable lag; in contrast, when UP-2 was pregrown on n-tetracosane and transferred to sodium acetate medium, a lag of 1 h was observed.
AB - The marine pseudomonad UP-2 grew, with a doubling time of 1.5 h at 30°C, in media supplemented with n-tetracosane as the sole organic carbon and energy source. Over 99% of the solid hydrocarbon was degraded. In order to overcome serious problems of sampling and measuring growth in the biphase system of hydrocarbon-water, a technique was developed combining the use of separate 1-ml eultures for each sample and32P labeling as an assay for growth. Using this technique, a growth durve of UP-2 on n-tetracosane was obtained. UP-2 pregrown on sodium acetate can utilize even-chain, liquid, or solid n-alkanes with no observable lag; in contrast, when UP-2 was pregrown on n-tetracosane and transferred to sodium acetate medium, a lag of 1 h was observed.
UR - http://www.scopus.com/inward/record.url?scp=0018609244&partnerID=8YFLogxK
U2 - 10.1007/BF02605875
DO - 10.1007/BF02605875
M3 - מאמר
AN - SCOPUS:0018609244
VL - 2
SP - 163
EP - 167
JO - Current Microbiology
JF - Current Microbiology
SN - 0343-8651
IS - 3
ER -