31P NMR and triple quantum filtered 23Na NMR studies of the effects of inhibition of Na+/H+ exchange on intracellular sodium and pH in working and ischemic hearts

Gil Navon, Jeffrey G. Werrmann, Ron Maron, Sheila M. Cohen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The triple quantum filtered 23Na NMR method is applied here to measure the effects of EIPA, a specific inhibitor of the Na+/H+antiporter, on relative intracellular sodium concentrations in isolated working hearts at baseline, during ischemia, and at subsequent reperfusion. In analogy to the spectrophotometric isosbestic point, an approach is developed that defines a value of τ at which the effect of the relaxation times on the TQF signal intensities is minimized, and the signals are proportional to the sodium concentration for both ischemic and working hearts. EIPA at 1.5 μ significantly inhibited (P < 0.01) the influx of intracellular Na+ during 20 min of ischemia at 36.2°C in this rat heart model. In parallel31P NMR studies, EIPA had no effect on either the development of acidosis during ischemia or on the recovery of pH, during reperfusion despite its profound effect on intracellular Na+ influx. Thus, under our conditions the Na+/H+ antiporter did not play a critical role in the maintenance of intracellular pH. EIPA treatment resulted in improved recovery (P < 0.005) of mechanical function after 20 min of ischemia. [ATP] was higher in treated hearts during ischemia and reperfusion.

Original languageEnglish
Pages (from-to)556-564
Number of pages9
JournalMagnetic Resonance in Medicine
Volume32
Issue number5
DOIs
StatePublished - Nov 1994

Keywords

  • Na NMR
  • Na/H exchange
  • P NMR
  • heart

Fingerprint

Dive into the research topics of '31P NMR and triple quantum filtered 23Na NMR studies of the effects of inhibition of Na+/H+ exchange on intracellular sodium and pH in working and ischemic hearts'. Together they form a unique fingerprint.

Cite this