Sulforhodamine GM1-ganglioside: synthesis and physico-chemical properties

S. Marchesini, L. Demasi, P. Cestone, A. Preti, V. Agmon, A. Dagan, R. Navon, S. Gatt

Research output: Contribution to journalArticlepeer-review

Abstract

A fluorescent derivative of GM1-ganglioside was synthesized by linking sulforhodamine 101 to the sphingosine moiety through amino dodecanoyl residue. The product (SR-12GM1) was quantitatively converted to SR-12GM2 by treatment with bovine testes β-galactosidase and in intact cultured human skin fibroblasts was catabolized to sulforhodamine GM2, GM3 and ceramide; the latter product was further converted to sphingomyelin. In aqueous medium SR-12GM1 formed micelles. When transfer from micelles to vesicles and between vesicles was compared with that of pyrene-GM1, the transfer of SR-12GM1 occurred at higher rates, following in both cases a biexponential curve.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalChemistry and Physics of Lipids
Volume72
Issue number2
DOIs
StatePublished - 8 Aug 1994

Keywords

  • Fluorescent gangliosides
  • GM-ganglioside
  • Gangliosides transfer
  • Sulforhodamine 101

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