TY - JOUR
T1 - Subzero nonfreezing cryopresevation of rat hearts using antifreeze protein I and antifreeze protein III
AU - Amir, Gabriel
AU - Horowitz, Liana
AU - Rubinsky, Boris
AU - Yousif, Basheer Sheick
AU - Lavee, Jacob
AU - Smolinsky, Aram K.
N1 - Funding Information:
This study was partially funded by an unconditional grant provided by A/F Protein, Inc. One of the authors (RB) has a commercial interest in A/F Protein, Inc.
PY - 2004/6
Y1 - 2004/6
N2 - The purpose of the present study was to evaluate whether AFPs protect the heart from freezing and improve survival and viability in subzero cryopreservation. Hearts were subject to 5 preservation protocols; University of Wisconsin solution (UW) at 4°C, UW at -1.3°C without nucleation, UW at -1.3°C with nucleation, UW AFP I (15mg/cm3) at -1.3°C with nucleation, and in UW AFP III (15mg/cm3) at -1.3°C with nucleation. Hearts were preserved for 24, 28, and 32h, rewarmed and connected to the working isolated perfusion system. Data [heart rate (HR), coronary flow (CF), and developed pressure (dP)] was collected 30 and 60min after reperfusion. Hearts preserved at -1.3°C without AFPs froze, while hearts preserved with AFP did not freeze when nucleation was initiated and survived. Survival and dP of hearts preserved for 24h at -1.3°C using AFP III was better than those preserved at 4°C, (dP; 1.4 vs. 0.8, p<0.05). Four of six hearts and six of six hearts died when preserved at 4°C for 28 and 32h, respectively, all of the hearts that were preserved at -1.3°C with or without AFPs survived after 28h (n=18) and 32h (n=18). CF was higher in UW -1.3°C group without attempted nucleation than in AFP I and AFP III groups after 28 and 32h (3.4 vs. 1.7, p<0.05, and 3.4 vs. 1.7, p<0.05, respectively). In conclusion, AFPs were found to protect the heart from freezing and improve survival and dP (AFP III) in prolonged subzero preservation.
AB - The purpose of the present study was to evaluate whether AFPs protect the heart from freezing and improve survival and viability in subzero cryopreservation. Hearts were subject to 5 preservation protocols; University of Wisconsin solution (UW) at 4°C, UW at -1.3°C without nucleation, UW at -1.3°C with nucleation, UW AFP I (15mg/cm3) at -1.3°C with nucleation, and in UW AFP III (15mg/cm3) at -1.3°C with nucleation. Hearts were preserved for 24, 28, and 32h, rewarmed and connected to the working isolated perfusion system. Data [heart rate (HR), coronary flow (CF), and developed pressure (dP)] was collected 30 and 60min after reperfusion. Hearts preserved at -1.3°C without AFPs froze, while hearts preserved with AFP did not freeze when nucleation was initiated and survived. Survival and dP of hearts preserved for 24h at -1.3°C using AFP III was better than those preserved at 4°C, (dP; 1.4 vs. 0.8, p<0.05). Four of six hearts and six of six hearts died when preserved at 4°C for 28 and 32h, respectively, all of the hearts that were preserved at -1.3°C with or without AFPs survived after 28h (n=18) and 32h (n=18). CF was higher in UW -1.3°C group without attempted nucleation than in AFP I and AFP III groups after 28 and 32h (3.4 vs. 1.7, p<0.05, and 3.4 vs. 1.7, p<0.05, respectively). In conclusion, AFPs were found to protect the heart from freezing and improve survival and dP (AFP III) in prolonged subzero preservation.
KW - Antifreeze proteins
KW - Cryopreservation
KW - Heart
KW - Preservation
KW - Subzero
UR - http://www.scopus.com/inward/record.url?scp=2442438608&partnerID=8YFLogxK
U2 - 10.1016/j.cryobiol.2004.02.009
DO - 10.1016/j.cryobiol.2004.02.009
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 15157776
AN - SCOPUS:2442438608
SN - 0011-2240
VL - 48
SP - 273
EP - 282
JO - Cryobiology
JF - Cryobiology
IS - 3
ER -