TY - JOUR
T1 - Subunit structure of hemoglobin from the clam shrimp Cyzicus
AU - David, Melvyn M.
AU - Schejter, Abel
AU - Daniel, Ezra
AU - Ben-Shaul, Yehuda
PY - 1977/4/5
Y1 - 1977/4/5
N2 - The subunit structure of hemoglobin from the clam shrimp Cyzicus was investigated. The native protein has a molecular weight of 280,000 as shown by sedimentation equilibrium. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of mercaptoethanol resulted in a single band corresponding to a molecular weight of 15,500. A molecular weight of 14,500 was determined by the method of sedimentation equilibrium for the isolated polypeptide chain. Electron microscopy of the protein revealed a pentagonal structure. It was concluded that the native molecule contains 20 polypeptide chains.
AB - The subunit structure of hemoglobin from the clam shrimp Cyzicus was investigated. The native protein has a molecular weight of 280,000 as shown by sedimentation equilibrium. Sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the presence of mercaptoethanol resulted in a single band corresponding to a molecular weight of 15,500. A molecular weight of 14,500 was determined by the method of sedimentation equilibrium for the isolated polypeptide chain. Electron microscopy of the protein revealed a pentagonal structure. It was concluded that the native molecule contains 20 polypeptide chains.
UR - http://www.scopus.com/inward/record.url?scp=0017483387&partnerID=8YFLogxK
U2 - 10.1016/S0022-2836(77)80125-3
DO - 10.1016/S0022-2836(77)80125-3
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AN - SCOPUS:0017483387
SN - 0022-2836
VL - 111
SP - 211
EP - 214
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 2
ER -