TY - JOUR
T1 - Subunit Structure and Biogenesis of ATP Synthase and Photosystem I Reaction Center
AU - Nelson, Nathan
N1 - Funding Information:
We thank Dr. Kenneth Leto (Dupont) for advice on the electrophoresis of chlorophyll-protein complexes and Ms. Sydell Lamb for expert assistance with raising antibodies. Research by R.S.W. at the University of Warwick is supported by a Postgraduate Studentship from the Science and Engineering Research Council (UK). L.E.S. is supported by a Graduate Council Fellowship through State University of New York at Stony Brook. Research at the Brookhaven National Laboratory is performed under the auspices of the United States Department of Energy.
PY - 1986/1
Y1 - 1986/1
N2 - This chapter describes the preparation of NaBr-treated chloroplasts, purification of photosystem I reaction center, preparation of antibodies to individual subunits, and methods for the study of the biogenesis of the Adenosine-5'-triphosphate (ATP) synthase and photosystem I reaction center. Multisubunit protein complexes are vital for the function of biological membranes. The chloroplast inner membrane contains four protein complexes that together with single subunit proteins catalyze light-dependent oxygen evolution, nicotinamide adenine dinucleotide phosphate (NADP) reduction, and adenosine diphosphate ADP photophosphorylation. The four protein complexes are photosystem I reaction center, photosystem II reaction center, cytochrome b6-f complex, and ATP synthase. A protein complex is the minimal structure that catalyzes a characterized biochemical reaction. Photosystem I reaction center is the minimal structure that catalyzes plastocyanin photooxidation and ferredoxin photoreduction. The purified photosystem I reaction center is active in NADP photoreduction.
AB - This chapter describes the preparation of NaBr-treated chloroplasts, purification of photosystem I reaction center, preparation of antibodies to individual subunits, and methods for the study of the biogenesis of the Adenosine-5'-triphosphate (ATP) synthase and photosystem I reaction center. Multisubunit protein complexes are vital for the function of biological membranes. The chloroplast inner membrane contains four protein complexes that together with single subunit proteins catalyze light-dependent oxygen evolution, nicotinamide adenine dinucleotide phosphate (NADP) reduction, and adenosine diphosphate ADP photophosphorylation. The four protein complexes are photosystem I reaction center, photosystem II reaction center, cytochrome b6-f complex, and ATP synthase. A protein complex is the minimal structure that catalyzes a characterized biochemical reaction. Photosystem I reaction center is the minimal structure that catalyzes plastocyanin photooxidation and ferredoxin photoreduction. The purified photosystem I reaction center is active in NADP photoreduction.
UR - http://www.scopus.com/inward/record.url?scp=0000763582&partnerID=8YFLogxK
U2 - 10.1016/0076-6879(86)18085-2
DO - 10.1016/0076-6879(86)18085-2
M3 - מאמר
AN - SCOPUS:0000763582
VL - 118
SP - 352
EP - 369
JO - Methods in Enzymology
JF - Methods in Enzymology
SN - 0076-6879
IS - C
ER -