SUBCELLULAR FRACTIONATION STUDIES RELATED TO THE PROCESSING OF NEUROSECRETORY PROTEINS IN APLYSIA NEURONS

Y. Peng Loh; Y. Sarne; M. P. Daniels; H. Gainer

doi:10.1111/j.1471-4159.1977.tb03935.x

SUBCELLULAR FRACTIONATION STUDIES RELATED TO THE PROCESSING OF NEUROSECRETORY PROTEINS IN APLYSIA NEURONS

Y. Peng Loh^*
, Y. Sarne
, M. P. Daniels
, H. Gainer

^*Corresponding author for this work

National Institutes of Health
National Heart

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Abstract— Neurosecretory cells (bag cells and R_3–14 neurons) in the abdominal ganglion of Aplysia californica were ‘pulse‐chased’ in [³H]leucine and comparisons of the labeled protein profiles from the total cell homogenate versus a crude ‘neurosecretory granule’ fraction on acid‐urea polyacrylamide gels were made, The data provides indirect support for the hypothesis that some of the post‐translational processing of the neurosecretory proteins occurs intragranularly (Lohet al, 1975). In the case of the Bag cells the initial processing of the 29,000 daltons precursor appears to occur extragranularly, possibly in the rough endoplasmic reticulum cisternae.

Original language	English
Pages (from-to)	135-139
Number of pages	5
Journal	Journal of Neurochemistry
Volume	29
Issue number	1
DOIs	https://doi.org/10.1111/j.1471-4159.1977.tb03935.x
State	Published - Jul 1977
Externally published	Yes

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Cite this

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title = "SUBCELLULAR FRACTIONATION STUDIES RELATED TO THE PROCESSING OF NEUROSECRETORY PROTEINS IN APLYSIA NEURONS",

abstract = "Abstract— Neurosecretory cells (bag cells and R3–14 neurons) in the abdominal ganglion of Aplysia californica were {\textquoteleft}pulse‐chased{\textquoteright} in [3H]leucine and comparisons of the labeled protein profiles from the total cell homogenate versus a crude {\textquoteleft}neurosecretory granule{\textquoteright} fraction on acid‐urea polyacrylamide gels were made, The data provides indirect support for the hypothesis that some of the post‐translational processing of the neurosecretory proteins occurs intragranularly (Lohet al, 1975). In the case of the Bag cells the initial processing of the 29,000 daltons precursor appears to occur extragranularly, possibly in the rough endoplasmic reticulum cisternae.",

author = "Loh, \{Y. Peng\} and Y. Sarne and Daniels, \{M. P.\} and H. Gainer",

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AU - Sarne, Y.

AU - Daniels, M. P.

AU - Gainer, H.

PY - 1977/7

Y1 - 1977/7

N2 - Abstract— Neurosecretory cells (bag cells and R3–14 neurons) in the abdominal ganglion of Aplysia californica were ‘pulse‐chased’ in [3H]leucine and comparisons of the labeled protein profiles from the total cell homogenate versus a crude ‘neurosecretory granule’ fraction on acid‐urea polyacrylamide gels were made, The data provides indirect support for the hypothesis that some of the post‐translational processing of the neurosecretory proteins occurs intragranularly (Lohet al, 1975). In the case of the Bag cells the initial processing of the 29,000 daltons precursor appears to occur extragranularly, possibly in the rough endoplasmic reticulum cisternae.

AB - Abstract— Neurosecretory cells (bag cells and R3–14 neurons) in the abdominal ganglion of Aplysia californica were ‘pulse‐chased’ in [3H]leucine and comparisons of the labeled protein profiles from the total cell homogenate versus a crude ‘neurosecretory granule’ fraction on acid‐urea polyacrylamide gels were made, The data provides indirect support for the hypothesis that some of the post‐translational processing of the neurosecretory proteins occurs intragranularly (Lohet al, 1975). In the case of the Bag cells the initial processing of the 29,000 daltons precursor appears to occur extragranularly, possibly in the rough endoplasmic reticulum cisternae.

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SUBCELLULAR FRACTIONATION STUDIES RELATED TO THE PROCESSING OF NEUROSECRETORY PROTEINS IN APLYSIA NEURONS

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