SUBCELLULAR FRACTIONATION STUDIES RELATED TO THE PROCESSING OF NEUROSECRETORY PROTEINS IN APLYSIA NEURONS

Y. Peng Loh, Y. Sarne, M. P. Daniels, H. Gainer

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract— Neurosecretory cells (bag cells and R3–14 neurons) in the abdominal ganglion of Aplysia californica were ‘pulse‐chased’ in [3H]leucine and comparisons of the labeled protein profiles from the total cell homogenate versus a crude ‘neurosecretory granule’ fraction on acid‐urea polyacrylamide gels were made, The data provides indirect support for the hypothesis that some of the post‐translational processing of the neurosecretory proteins occurs intragranularly (Lohet al, 1975). In the case of the Bag cells the initial processing of the 29,000 daltons precursor appears to occur extragranularly, possibly in the rough endoplasmic reticulum cisternae.

Original languageEnglish
Pages (from-to)135-139
Number of pages5
JournalJournal of Neurochemistry
Volume29
Issue number1
DOIs
StatePublished - Jul 1977
Externally publishedYes

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