Sub-second, super-resolved imaging of biological systems using parallel EO-STED

Arik Girsault, Amit Meller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


We present a parallel stimulated emission depletion (STED) nanoscope with no mechanical moving parts and sub-millisecond pixel dwell times, relying on electro-optical (EO) phase modulators. The nanoscope offers 1225-fold parallelization over single-doughnut-scanning STED and achieves a spatial resolution of 35 nm. We imaged immunostained nuclear pore complexes of zebrafish within their natural biological environment, demonstrating spatial and temporal resolutions of 56 nm and 0.2 s, respectively. Furthermore, we show parallel EO-STED sub-second imaging of microtubules inside living cells. Finally, we reveal the nanodomain organization of a eukaryotic initiation factor within the processing bodies of fixed cells. The potential of parallel EO-STED to offer microsecond pixel dwell times over large fields of view promises millisecond STED imaging.

Original languageEnglish
Pages (from-to)2712-2715
Number of pages4
JournalOptics Letters
Issue number10
StatePublished - 15 May 2020
Externally publishedYes


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