TY - JOUR
T1 - Study of the semi ordered water in an active site by time resolved measurement of a single diffusing proton
AU - Gutman, Menachem
AU - Nachliel, Esther
N1 - Funding Information:
The research was supported by the Office of Naval
PY - 1993/5
Y1 - 1993/5
N2 - The reaction between photochemically generated ion pair, H+ and excited anion, was monitored by picosecond fluorescence dynamics. The reaction measured in bulk water was compared with that proceeding inside specific binding sites of two proteins, apomyoglobin and the anionic specific channel, porine of E. coli. The results indicate that the water in the sites deviates markedly from the liquid state in the bulk, having a lower dielectric constant and a smaller diffusivity of proton. Both terms point to a partial ordering of the water in the immediate vicinity of a protein.
AB - The reaction between photochemically generated ion pair, H+ and excited anion, was monitored by picosecond fluorescence dynamics. The reaction measured in bulk water was compared with that proceeding inside specific binding sites of two proteins, apomyoglobin and the anionic specific channel, porine of E. coli. The results indicate that the water in the sites deviates markedly from the liquid state in the bulk, having a lower dielectric constant and a smaller diffusivity of proton. Both terms point to a partial ordering of the water in the immediate vicinity of a protein.
UR - http://www.scopus.com/inward/record.url?scp=0027597019&partnerID=8YFLogxK
U2 - 10.1016/0167-2738(93)90358-A
DO - 10.1016/0167-2738(93)90358-A
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0027597019
SN - 0167-2738
VL - 61
SP - 229
EP - 234
JO - Solid State Ionics
JF - Solid State Ionics
IS - 1-3
ER -