TY - JOUR
T1 - Study of hypothermia on cultured neuronal networks using multi-electrode arrays
AU - Rubinsky, Liel
AU - Raichman, Nadav
AU - Baruchi, Itay
AU - Shein, Mark
AU - Lavee, Jacob
AU - Frenk, Hanan
AU - Ben-Jacob, Eshel
PY - 2007/3/15
Y1 - 2007/3/15
N2 - Efficient and safe use of hypothermia during various neuro-medical procedures requires sound understanding of low temperature effects on the neuronal network's activity. In this report, we introduce the use of cultivated dissociated neuronal networks on temperature controlled multi-electrode arrays (MEAs) as a simple methodology for studying the long-term effects of hypothermia. The networks exhibit spontaneous activity in the form of synchronized bursting events (SBEs), followed by long intervals of sporadic firing. Through the use of our correlation method, these SBEs can be clustered into sub-groups of similar spatio-temporal patterns. Application of hypothermia to the network resulted in a reduction in the SBE rate, the spike intensity and an increase in inter-neuronal correlations. Within 2 h following the cessation of hypothermia, the cultured network returned to its initial spatio-temporal SBE structure. These results suggest that the network survived cold exposure and demonstrate the feasibility of long-term continuous neural network recording during hypothermic conditions.
AB - Efficient and safe use of hypothermia during various neuro-medical procedures requires sound understanding of low temperature effects on the neuronal network's activity. In this report, we introduce the use of cultivated dissociated neuronal networks on temperature controlled multi-electrode arrays (MEAs) as a simple methodology for studying the long-term effects of hypothermia. The networks exhibit spontaneous activity in the form of synchronized bursting events (SBEs), followed by long intervals of sporadic firing. Through the use of our correlation method, these SBEs can be clustered into sub-groups of similar spatio-temporal patterns. Application of hypothermia to the network resulted in a reduction in the SBE rate, the spike intensity and an increase in inter-neuronal correlations. Within 2 h following the cessation of hypothermia, the cultured network returned to its initial spatio-temporal SBE structure. These results suggest that the network survived cold exposure and demonstrate the feasibility of long-term continuous neural network recording during hypothermic conditions.
KW - Hypothermia
KW - Multi-electrode arrays
KW - Neuronal networks
KW - Synchronized bursting events
UR - http://www.scopus.com/inward/record.url?scp=33846929104&partnerID=8YFLogxK
U2 - 10.1016/j.jneumeth.2006.09.017
DO - 10.1016/j.jneumeth.2006.09.017
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C2 - 17081617
AN - SCOPUS:33846929104
SN - 0165-0270
VL - 160
SP - 288
EP - 293
JO - Journal of Neuroscience Methods
JF - Journal of Neuroscience Methods
IS - 2
ER -