Study of hypothermia on cultured neuronal networks using multi-electrode arrays

Liel Rubinsky*, Nadav Raichman, Itay Baruchi, Mark Shein, Jacob Lavee, Hanan Frenk, Eshel Ben-Jacob

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Efficient and safe use of hypothermia during various neuro-medical procedures requires sound understanding of low temperature effects on the neuronal network's activity. In this report, we introduce the use of cultivated dissociated neuronal networks on temperature controlled multi-electrode arrays (MEAs) as a simple methodology for studying the long-term effects of hypothermia. The networks exhibit spontaneous activity in the form of synchronized bursting events (SBEs), followed by long intervals of sporadic firing. Through the use of our correlation method, these SBEs can be clustered into sub-groups of similar spatio-temporal patterns. Application of hypothermia to the network resulted in a reduction in the SBE rate, the spike intensity and an increase in inter-neuronal correlations. Within 2 h following the cessation of hypothermia, the cultured network returned to its initial spatio-temporal SBE structure. These results suggest that the network survived cold exposure and demonstrate the feasibility of long-term continuous neural network recording during hypothermic conditions.

Original languageEnglish
Pages (from-to)288-293
Number of pages6
JournalJournal of Neuroscience Methods
Issue number2
StatePublished - 15 Mar 2007


  • Hypothermia
  • Multi-electrode arrays
  • Neuronal networks
  • Synchronized bursting events


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