Study of differentiation of fresh myelogenous leukemic cells by compounds that induce a human promyelocytic leukemic line (HL-60) to differentiate

H. Phillip Koeffler*, Leslie Yelton, Miron Prokocimer, Karim Hirji

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The human promyelocytic leukemia cell line known as HL-60 can be triggered to mature to functional granulocytes and/or macrophages after exposure to a variety of compounds. The findings have generated enthusiasm for possible therapy of leukemia using compounds that induce leukemic cell differentiation. We investigated whether five compounds known to trigger HL-60 differentiation to granulocytes could trigger the maturation of blast cells from 12 patients with myelogenous leukemia. Maturation was judged by morphology, superoxide production, phagocytosis, expression of Fc receptors, and development of α-napthyl acetate esterase activity. The blast cells from most patients showed little morphological, histological or functional maturation after exposure to the various compounds as compared to the blast cells cultured without the compounds. Actinomycin was able to induce significant maturation of leukemic cells of some patients when maturation was analyzed by several statistical methods. Our study suggests that many compounds which trigger differentiation of promyelocytic leukemia cells may not trigger differentiation of less mature myeloid leukemic cells.

Original languageEnglish
Pages (from-to)73-81
Number of pages9
JournalLeukemia Research
Volume9
Issue number1
DOIs
StatePublished - 1985
Externally publishedYes

Keywords

  • Promyelocytic leukemic cells
  • human myeloid differentiation
  • myelogenous leukemia

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