TY - JOUR
T1 - Studies on the mechanism of fatty acid synthesis III. Products of enzymic synthesis of fatty acids
AU - Porter, John W.
AU - Tietz, Alisa
N1 - Funding Information:
* This work has been supported by a grant-in-aid trom the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council; by a research grant, H-2236, and a postdoctoral training grant HTS-5oo6, from the National Heart Institute of the National Institutes of Health, Public Health Service; and aided by a contract between the Office of Naval Research, Department of the Navy, and the University of Wisconsin, NR 12o-264. Preliminary accounts of this investigation have been reported t,2. * * Postdoctoral Trainee of the University of Wisconsin, Institute for Enzyme Research. Present address: Veterans Hospital and Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin. * ** Postdoctoral Trainee of the University of Wisconsin, institute for Enzyme Research. Present address: Department of Biochemistry, College of Medicine, New York University, New York City.
PY - 1957
Y1 - 1957
N2 - The long-chain fatty acids synthesized from acetate by an enzyme system reconstructed fom soluble fractions of pigeon liver have been separated and identified by a variety of techniques. Free palmitic acid and progressively smaller amounts of free myristic, lauric and decanoic acids are principally formed in this system. Small quantities of esterified long-chain acids with the properties of palmityl CoA were also found. Short-chain fatty acids do not accumulate, but small amounts of the hydroxamates of short-chain acids can be formed if acetyl CoA is used as substrate in place of acetate. HMG has been identified in the incubation mixture. Net synthesis of fatty acids was demonstrated by direct weighing, titration and radioactivity measurements. The specific radioactivity of the carboxyl carbon of fatty acids synthesized from carboxyl-labeled acetate was 1.7 times that of the average carbon atom in the entire chain. This finding is in agreement with the concept of fatty acid synthesis via head-to-tail condensations of acetate units.
AB - The long-chain fatty acids synthesized from acetate by an enzyme system reconstructed fom soluble fractions of pigeon liver have been separated and identified by a variety of techniques. Free palmitic acid and progressively smaller amounts of free myristic, lauric and decanoic acids are principally formed in this system. Small quantities of esterified long-chain acids with the properties of palmityl CoA were also found. Short-chain fatty acids do not accumulate, but small amounts of the hydroxamates of short-chain acids can be formed if acetyl CoA is used as substrate in place of acetate. HMG has been identified in the incubation mixture. Net synthesis of fatty acids was demonstrated by direct weighing, titration and radioactivity measurements. The specific radioactivity of the carboxyl carbon of fatty acids synthesized from carboxyl-labeled acetate was 1.7 times that of the average carbon atom in the entire chain. This finding is in agreement with the concept of fatty acid synthesis via head-to-tail condensations of acetate units.
UR - http://www.scopus.com/inward/record.url?scp=4243569011&partnerID=8YFLogxK
U2 - 10.1016/0006-3002(57)90414-6
DO - 10.1016/0006-3002(57)90414-6
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AN - SCOPUS:4243569011
SN - 0006-3002
VL - 25
SP - 41
EP - 50
JO - BBA - Biochimica et Biophysica Acta
JF - BBA - Biochimica et Biophysica Acta
IS - C
ER -