TY - JOUR
T1 - Studies on the mechanism of fatty acid synthesis II. Cofactor requirements of the soluble pigeon liver system
AU - Porter, John W.
AU - Wakil, Salih J.
AU - Tietz, Alisa
AU - Jacob, Miriam I.
AU - Gibson, David M.
N1 - Funding Information:
In the first paper of this series 9 the preparation and purification of a soluble enzyme system from pigeon liver which actively synthesizes long-chain fatty acids from acetate was described. Four enzyme fractions were prepared which only on recombination catalyzed fatty acid synthesis. The present paper describes in detail the cofactor requirements for synthesis. With the most purified enzyme fractions tile following cofactors are necessary: ATP, CoA, DPNH, TPN, Mn ++, isocitrate and a suitable sulfhydryl compound such as glutathione or cysteine. In the unfractionated "This work has been supported by a grant-in-aid from the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council; by a research grant, H-2236, and a postdoctoral training grant, HTS-5oo6, from the National Heart Institute of tile National Institutes of Ilealth, Public Health Service; and aided by a contract between the Office of Naval Research, Department of the Navy, and the Univers£tv of Wisconsin, NR t2o-264. Preliminary accounts of this investigation have been reported 1,2,~.i. * * Postdoctoral Trainee of the University of Wisconsin, Institute for Enzyme Research. Present address : Veterans Hospital and Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin. "" * t'ostdoctoral Trainee of the L'niversity of Wisconsin, Institute for Enzyme Research. Present address: Department of Biochemistry, College of Medicine, New York University, New York City. § Postdoctoral Trainee of the University of Wisconsin, Institute for Enzyme Research. Present address: Department of Physiology, School of Medicine, Western Reserve University, Cleveland,
Funding Information:
* This work has been supported by a grant-in-aid trom the American Cancer Society upon recommendation of the Committee on Growth of the National Research Council; by a research grant, H-2236, and a postdoctoral training grant HTS-5oo6, from the National Heart Institute of the National Institutes of Health, Public Health Service; and aided by a contract between the Office of Naval Research, Department of the Navy, and the University of Wisconsin, NR 12o-264. Preliminary accounts of this investigation have been reported t,2. * * Postdoctoral Trainee of the University of Wisconsin, Institute for Enzyme Research. Present address: Veterans Hospital and Department of Physiological Chemistry, University of Wisconsin, Madison, Wisconsin. * ** Postdoctoral Trainee of the University of Wisconsin, institute for Enzyme Research. Present address: Department of Biochemistry, College of Medicine, New York University, New York City.
PY - 1957
Y1 - 1957
N2 - The synthesis of long-chain fatty acids from acetate by the reconstituted fractions of pigeon liver supernatant requires the following cofactors: ATP, CoA, GSH, DPNH, Mn++, isocitrate and TPN. Glucose-i-phosphate is needed in crude systems but this component can be replaced by DPNH in the more purified system. Isocitrate cannot be replaced by TPNH or a TPNH-generating system such as glucose-6-phosphate dehydrogenase system plus substrate. However, it can be replaced by a combination of α-ketoglutarate, CO2, and a TPNH generating system.
AB - The synthesis of long-chain fatty acids from acetate by the reconstituted fractions of pigeon liver supernatant requires the following cofactors: ATP, CoA, GSH, DPNH, Mn++, isocitrate and TPN. Glucose-i-phosphate is needed in crude systems but this component can be replaced by DPNH in the more purified system. Isocitrate cannot be replaced by TPNH or a TPNH-generating system such as glucose-6-phosphate dehydrogenase system plus substrate. However, it can be replaced by a combination of α-ketoglutarate, CO2, and a TPNH generating system.
UR - http://www.scopus.com/inward/record.url?scp=4243500625&partnerID=8YFLogxK
U2 - 10.1016/0006-3002(57)90413-4
DO - 10.1016/0006-3002(57)90413-4
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AN - SCOPUS:4243500625
SN - 0006-3002
VL - 25
SP - 35
EP - 41
JO - BBA - Biochimica et Biophysica Acta
JF - BBA - Biochimica et Biophysica Acta
IS - C
ER -