@article{9c6167351bc74faca28cd419a4d59f75,
title = "Structure of plant photosystem I-plastocyanin complex reveals strong hydrophobic interactions",
abstract = "Photosystem I is defined as plastocyanin-ferredoxin oxidoreductase. Taking advantage of genetic engineering, kinetic analyses and cryo-EM, our data provide novel mechanistic insights into binding and electron transfer between PSI and Pc. Structural data at 2.74 {\AA} resolution reveals strong hydrophobic interactions in the plant PSI-Pc ternary complex, leading to exclusion of water molecules from PsaA-PsaB/Pc interface once the PSI-Pc complex forms. Upon oxidation of Pc, a slight tilt of bound oxidized Pc allows water molecules to accommodate the space between Pc and PSI to drive Pc dissociation. Such a scenario is consistent with the six times larger dissociation constant of oxidized as compared with reduced Pc and mechanistically explains how this molecular machine optimized electron transfer for fast turnover.",
author = "Ido Caspy and Mariia Fadeeva and Sebastian Kuhlgert and Anna Borovikova-Sheinker and Daniel Klaiman and Gal Masrati and Friedel Drepper and Nir Ben-Tal and Michael Hippler and Nathan Nelson",
note = "Publisher Copyright: {\textcopyright} 2021 The Author(s).",
year = "2021",
month = jun,
doi = "10.1042/BCJ20210267",
language = "אנגלית",
volume = "478",
pages = "2371--2384",
journal = "Biochemical Journal",
issn = "0264-6021",
publisher = "Portland Press Ltd.",
number = "12",
}