Structure of extracellular hemoglobin from the brine shrimp Artemia salina

Abdussalam Azem; Ezra Daniel

doi:10.1016/0305-0491(92)90176-R

Structure of extracellular hemoglobin from the brine shrimp Artemia salina

Abdussalam Azem, Ezra Daniel

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

1. 1. Hemoglobin from the brine shrimp Artemia salina, purified by ultracentrifugation and preparative gel electrophoresis in non-denaturing medium, gave in sodium dodecyl sulfate-polyacrylamide gel electrophoresis a single band corresponding to a polypeptide chain with M_r 150,000. 2. 2. Crosslinking by glutardialdehyde resulted in the appearance of a band corresponding to a molecular mass twice that of a polypeptide chain. 3. 3. Limited trypsinolysis gave eight proteolytic bands corresponding to submultiples 8 9- 1 9 of a polypeptide chain. 4. 4. We conclude that a molecular of Artemia hemoglobin is composed of two single polypeptide chain subunits and that each subunits consists of nine structural units roughly equal in size.

Original language	English
Pages (from-to)	185-188
Number of pages	4
Journal	Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume	101
Issue number	1-2
DOIs	https://doi.org/10.1016/0305-0491(92)90176-R
State	Published - 1992

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title = "Structure of extracellular hemoglobin from the brine shrimp Artemia salina",

abstract = "1. 1. Hemoglobin from the brine shrimp Artemia salina, purified by ultracentrifugation and preparative gel electrophoresis in non-denaturing medium, gave in sodium dodecyl sulfate-polyacrylamide gel electrophoresis a single band corresponding to a polypeptide chain with Mr 150,000. 2. 2. Crosslinking by glutardialdehyde resulted in the appearance of a band corresponding to a molecular mass twice that of a polypeptide chain. 3. 3. Limited trypsinolysis gave eight proteolytic bands corresponding to submultiples 8 9- 1 9 of a polypeptide chain. 4. 4. We conclude that a molecular of Artemia hemoglobin is composed of two single polypeptide chain subunits and that each subunits consists of nine structural units roughly equal in size.",

author = "Abdussalam Azem and Ezra Daniel",

year = "1992",

doi = "10.1016/0305-0491(92)90176-R",

language = "אנגלית",

volume = "101",

pages = "185--188",

journal = "Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology",

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publisher = "Elsevier Inc.",

number = "1-2",

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T1 - Structure of extracellular hemoglobin from the brine shrimp Artemia salina

AU - Azem, Abdussalam

AU - Daniel, Ezra

PY - 1992

Y1 - 1992

N2 - 1. 1. Hemoglobin from the brine shrimp Artemia salina, purified by ultracentrifugation and preparative gel electrophoresis in non-denaturing medium, gave in sodium dodecyl sulfate-polyacrylamide gel electrophoresis a single band corresponding to a polypeptide chain with Mr 150,000. 2. 2. Crosslinking by glutardialdehyde resulted in the appearance of a band corresponding to a molecular mass twice that of a polypeptide chain. 3. 3. Limited trypsinolysis gave eight proteolytic bands corresponding to submultiples 8 9- 1 9 of a polypeptide chain. 4. 4. We conclude that a molecular of Artemia hemoglobin is composed of two single polypeptide chain subunits and that each subunits consists of nine structural units roughly equal in size.

AB - 1. 1. Hemoglobin from the brine shrimp Artemia salina, purified by ultracentrifugation and preparative gel electrophoresis in non-denaturing medium, gave in sodium dodecyl sulfate-polyacrylamide gel electrophoresis a single band corresponding to a polypeptide chain with Mr 150,000. 2. 2. Crosslinking by glutardialdehyde resulted in the appearance of a band corresponding to a molecular mass twice that of a polypeptide chain. 3. 3. Limited trypsinolysis gave eight proteolytic bands corresponding to submultiples 8 9- 1 9 of a polypeptide chain. 4. 4. We conclude that a molecular of Artemia hemoglobin is composed of two single polypeptide chain subunits and that each subunits consists of nine structural units roughly equal in size.

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U2 - 10.1016/0305-0491(92)90176-R

DO - 10.1016/0305-0491(92)90176-R

M3 - מאמר

AN - SCOPUS:0026542586

VL - 101

SP - 185

EP - 188

JO - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

JF - Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology

SN - 1096-4959

IS - 1-2

ER -

Structure of extracellular hemoglobin from the brine shrimp Artemia salina

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