Structure of a minimal photosystem I from the green alga Dunaliella salina

Annemarie Perez-Boerema, Daniel Klaiman, Ido Caspy, Sigal Y. Netzer-El, Alexey Amunts*, Nathan Nelson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Solar energy harnessed by oxygenic photosynthesis supports most of the life forms on Earth. In eukaryotes, photosynthesis occurs in chloroplasts and is achieved by membrane-embedded macromolecular complexes that contain core and peripheral antennae with multiple pigments. The structure of photosystem I (PSI) comprises the core and light-harvesting (LHCI) complexes, which together form PSI–LHCI. Here we determined the structure of PSI–LHCI from the salt-tolerant green alga Dunaliella salina using X-ray crystallography and electron cryo-microscopy. Our results reveal a previously undescribed configuration of the PSI core. It is composed of only 7 subunits, compared with 14–16 subunits in plants and the alga Chlamydomonas reinhardtii, and forms the smallest known PSI. The LHCI is poorly conserved at the sequence level and binds to pigments that form new energy pathways, and the interactions between the individual Lhca1–4 proteins are weakened. Overall, the data indicate the PSI of D. salina represents a different type of the molecular organization that provides important information for reconstructing the plasticity and evolution of PSI.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalNature Plants
Issue number3
StatePublished - 1 Mar 2020


FundersFunder number
Ragnar Söderberg FoundationM44/16
H2020 European Research Council
European Research CouncilERC-2018-StG-805230
Stiftelsen för Strategisk ForskningFFL15:0325
German-Israeli Foundation for Scientific Research and Development1483
Israel Science Foundation569/17
Knut och Alice Wallenbergs Stiftelse2018.0080


    Dive into the research topics of 'Structure of a minimal photosystem I from the green alga Dunaliella salina'. Together they form a unique fingerprint.

    Cite this