@article{3844623fe3c74866881c5c2877c7ef9a,
title = "Structure-function studies of the G-domain from human gem, a novel small G-protein",
abstract = "Gem, a member of the Rad,Gem/Kir subfamily of small G-proteins, has unique sequence features. We report here the crystallographic structure determination of the Gem G-domain in complex with nucleotide to 2.4 {\AA} resolution. Although the basic Ras protein fold is maintained, the Gem switch regions emphatically differ from the Ras paradigm. Our ensuing biochemical characterization indicates that Gem G-domain markedly prefers GDP over GTP. Two known functions of Gem are distinctly affected by spatially separated clusters of mutations.",
keywords = "Calcium channel, Crystallography, Cytoskeleton, G-protein, Nucleotide",
author = "Yarden Opatowsky and Yehezkel Sasson and Isabella Shaked and Yvona Ward and Orna Chomsky-Hecht and Yael Litvak and Zvi Selinger and Kathleen Kelly and Hirsch, {Joel A.}",
note = "Funding Information: Thanks go to the staff of ID14-4 (ESRF, France) for assistance with diffraction experiments. We thank Doug Andres for generously sharing Cav β expression constructs. Preliminary studies were supported by a grant from the US–Israel Binational Science Foundation (JAH, KK). Continuing work has been funded by a grant to JAH from the Israel Cancer Research Fund and by NIH grant EY 03529 to ZS. Appendix A ",
year = "2006",
month = oct,
day = "30",
doi = "10.1016/j.febslet.2006.09.067",
language = "אנגלית",
volume = "580",
pages = "5959--5964",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Wiley-Blackwell",
number = "25",
}