Structure-function studies of the G-domain from human gem, a novel small G-protein

Yarden Opatowsky, Yehezkel Sasson, Isabella Shaked, Yvona Ward, Orna Chomsky-Hecht, Yael Litvak, Zvi Selinger, Kathleen Kelly, Joel A. Hirsch*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


Gem, a member of the Rad,Gem/Kir subfamily of small G-proteins, has unique sequence features. We report here the crystallographic structure determination of the Gem G-domain in complex with nucleotide to 2.4 Å resolution. Although the basic Ras protein fold is maintained, the Gem switch regions emphatically differ from the Ras paradigm. Our ensuing biochemical characterization indicates that Gem G-domain markedly prefers GDP over GTP. Two known functions of Gem are distinctly affected by spatially separated clusters of mutations.

Original languageEnglish
Pages (from-to)5959-5964
Number of pages6
JournalFEBS Letters
Issue number25
StatePublished - 30 Oct 2006


FundersFunder number
National Institutes of Health
National Eye InstituteR01EY003529
Israel Cancer Research Fund
United States-Israel Binational Science Foundation


    • Calcium channel
    • Crystallography
    • Cytoskeleton
    • G-protein
    • Nucleotide


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