TY - JOUR
T1 - Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor
AU - Gozes, I.
AU - Bodner, M.
AU - Shani, Y.
AU - Fridkin, M.
N1 - Funding Information:
We particularly acknowledge the excellent participation of Einat Bril in the sequencing project. We also thank Rina Avidor and Eliezer Giladi for discussing the results with us. Special thanks are due to Prof. Maynard Makman for critical reviewing of this manuscript, and Rona Levin for typing and editing. We are also grateful to interpharm Inc. for supporting M.F. This work was supported by grants (to I.G.) from the National Institute of Neurological and Communicative Disorders and Stroke (R01 NS 19860), the United States-lsrael Binational Science Foundation, and the Leo and Julia Forchheimer Center for Molecular Genetics and by a Bergmann Memorial Prize to I.G., who is the incumbent of the Samuel O. Freedman Career Development Chair in Cancer Research in perpetuity, established by the Montreal Chapter, Canadian Sociely for the Weizmann Institute of Science. We would like to thank Dr. Richard Goodman for making his human VIP sequence awfilable to us prior to publication.
PY - 1986
Y1 - 1986
N2 - To identify the VIP biosynthetic pathways, we have isolated the human VIP gene, using synthetic oligodeoxynucleotides. These specific hybridization probes were constructed according to the neuroblastoma VIP-cDNA sequence and contained up to 39 bases. The gene structure was deduced by direct chemical nucleotide sequencing. Six exons were thus far discovered; among them two short exons, one encoding VIP and the second encoding PHM-27 (a peptide having a N-terminal histidine and C-terminal methionine amide, closely related in sequence and activity to VIP). As a model system for VIP gene expression, we used a human buccal tumor producing elevated amounts of VIP. In these cells, a major transcript of the VIP-gene was identified as a long RNA containing intron sequences. The occurrence of elevated quantities of a high molecular weight, intron containing, gene transcript which is not processed directly into mature RNA suggests that VIP gene expression may be regulated at the RNA processing level.
AB - To identify the VIP biosynthetic pathways, we have isolated the human VIP gene, using synthetic oligodeoxynucleotides. These specific hybridization probes were constructed according to the neuroblastoma VIP-cDNA sequence and contained up to 39 bases. The gene structure was deduced by direct chemical nucleotide sequencing. Six exons were thus far discovered; among them two short exons, one encoding VIP and the second encoding PHM-27 (a peptide having a N-terminal histidine and C-terminal methionine amide, closely related in sequence and activity to VIP). As a model system for VIP gene expression, we used a human buccal tumor producing elevated amounts of VIP. In these cells, a major transcript of the VIP-gene was identified as a long RNA containing intron sequences. The occurrence of elevated quantities of a high molecular weight, intron containing, gene transcript which is not processed directly into mature RNA suggests that VIP gene expression may be regulated at the RNA processing level.
KW - VIP gene
KW - VIP-RNA
UR - https://www.scopus.com/pages/publications/0022522196
U2 - 10.1016/0196-9781(86)90156-7
DO - 10.1016/0196-9781(86)90156-7
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AN - SCOPUS:0022522196
SN - 0196-9781
VL - 7
SP - 1
EP - 6
JO - Peptides
JF - Peptides
IS - SUPPL. 1
ER -
