Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor

I. Gozes; M. Bodner; Y. Shani; M. Fridkin

doi:10.1016/0196-9781(86)90156-7

Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor

I. Gozes^*
, M. Bodner
, Y. Shani
, M. Fridkin

^*Corresponding author for this work

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

To identify the VIP biosynthetic pathways, we have isolated the human VIP gene, using synthetic oligodeoxynucleotides. These specific hybridization probes were constructed according to the neuroblastoma VIP-cDNA sequence and contained up to 39 bases. The gene structure was deduced by direct chemical nucleotide sequencing. Six exons were thus far discovered; among them two short exons, one encoding VIP and the second encoding PHM-27 (a peptide having a N-terminal histidine and C-terminal methionine amide, closely related in sequence and activity to VIP). As a model system for VIP gene expression, we used a human buccal tumor producing elevated amounts of VIP. In these cells, a major transcript of the VIP-gene was identified as a long RNA containing intron sequences. The occurrence of elevated quantities of a high molecular weight, intron containing, gene transcript which is not processed directly into mature RNA suggests that VIP gene expression may be regulated at the RNA processing level.

Original language	English
Pages (from-to)	1-6
Number of pages	6
Journal	Peptides
Volume	7
Issue number	SUPPL. 1
DOIs	https://doi.org/10.1016/0196-9781(86)90156-7
State	Published - 1986
Externally published	Yes

Funding

Funders	Funder number
Center for Molecular Genetics
National Institute of Neurological Disorders and Stroke	R01 NS 19860
Weizmann Institute of Science
United States-Israel Binational Science Foundation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

VIP gene
VIP-RNA

Access to Document

10.1016/0196-9781(86)90156-7

Cite this

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title = "Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor",

abstract = "To identify the VIP biosynthetic pathways, we have isolated the human VIP gene, using synthetic oligodeoxynucleotides. These specific hybridization probes were constructed according to the neuroblastoma VIP-cDNA sequence and contained up to 39 bases. The gene structure was deduced by direct chemical nucleotide sequencing. Six exons were thus far discovered; among them two short exons, one encoding VIP and the second encoding PHM-27 (a peptide having a N-terminal histidine and C-terminal methionine amide, closely related in sequence and activity to VIP). As a model system for VIP gene expression, we used a human buccal tumor producing elevated amounts of VIP. In these cells, a major transcript of the VIP-gene was identified as a long RNA containing intron sequences. The occurrence of elevated quantities of a high molecular weight, intron containing, gene transcript which is not processed directly into mature RNA suggests that VIP gene expression may be regulated at the RNA processing level.",

keywords = "VIP gene, VIP-RNA",

author = "I. Gozes and M. Bodner and Y. Shani and M. Fridkin",

note = "Funding Information: We particularly acknowledge the excellent participation of Einat Bril in the sequencing project. We also thank Rina Avidor and Eliezer Giladi for discussing the results with us. Special thanks are due to Prof. Maynard Makman for critical reviewing of this manuscript, and Rona Levin for typing and editing. We are also grateful to interpharm Inc. for supporting M.F. This work was supported by grants (to I.G.) from the National Institute of Neurological and Communicative Disorders and Stroke (R01 NS 19860), the United States-lsrael Binational Science Foundation, and the Leo and Julia Forchheimer Center for Molecular Genetics and by a Bergmann Memorial Prize to I.G., who is the incumbent of the Samuel O. Freedman Career Development Chair in Cancer Research in perpetuity, established by the Montreal Chapter, Canadian Sociely for the Weizmann Institute of Science. We would like to thank Dr. Richard Goodman for making his human VIP sequence awfilable to us prior to publication.",

year = "1986",

doi = "10.1016/0196-9781(86)90156-7",

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journal = "Peptides",

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TY - JOUR

T1 - Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor

AU - Gozes, I.

AU - Bodner, M.

AU - Shani, Y.

AU - Fridkin, M.

N1 - Funding Information: We particularly acknowledge the excellent participation of Einat Bril in the sequencing project. We also thank Rina Avidor and Eliezer Giladi for discussing the results with us. Special thanks are due to Prof. Maynard Makman for critical reviewing of this manuscript, and Rona Levin for typing and editing. We are also grateful to interpharm Inc. for supporting M.F. This work was supported by grants (to I.G.) from the National Institute of Neurological and Communicative Disorders and Stroke (R01 NS 19860), the United States-lsrael Binational Science Foundation, and the Leo and Julia Forchheimer Center for Molecular Genetics and by a Bergmann Memorial Prize to I.G., who is the incumbent of the Samuel O. Freedman Career Development Chair in Cancer Research in perpetuity, established by the Montreal Chapter, Canadian Sociely for the Weizmann Institute of Science. We would like to thank Dr. Richard Goodman for making his human VIP sequence awfilable to us prior to publication.

PY - 1986

Y1 - 1986

N2 - To identify the VIP biosynthetic pathways, we have isolated the human VIP gene, using synthetic oligodeoxynucleotides. These specific hybridization probes were constructed according to the neuroblastoma VIP-cDNA sequence and contained up to 39 bases. The gene structure was deduced by direct chemical nucleotide sequencing. Six exons were thus far discovered; among them two short exons, one encoding VIP and the second encoding PHM-27 (a peptide having a N-terminal histidine and C-terminal methionine amide, closely related in sequence and activity to VIP). As a model system for VIP gene expression, we used a human buccal tumor producing elevated amounts of VIP. In these cells, a major transcript of the VIP-gene was identified as a long RNA containing intron sequences. The occurrence of elevated quantities of a high molecular weight, intron containing, gene transcript which is not processed directly into mature RNA suggests that VIP gene expression may be regulated at the RNA processing level.

AB - To identify the VIP biosynthetic pathways, we have isolated the human VIP gene, using synthetic oligodeoxynucleotides. These specific hybridization probes were constructed according to the neuroblastoma VIP-cDNA sequence and contained up to 39 bases. The gene structure was deduced by direct chemical nucleotide sequencing. Six exons were thus far discovered; among them two short exons, one encoding VIP and the second encoding PHM-27 (a peptide having a N-terminal histidine and C-terminal methionine amide, closely related in sequence and activity to VIP). As a model system for VIP gene expression, we used a human buccal tumor producing elevated amounts of VIP. In these cells, a major transcript of the VIP-gene was identified as a long RNA containing intron sequences. The occurrence of elevated quantities of a high molecular weight, intron containing, gene transcript which is not processed directly into mature RNA suggests that VIP gene expression may be regulated at the RNA processing level.

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KW - VIP-RNA

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ER -

Structure and expression of the vasoactive intestinal peptide (VIP) gene in a human tumor

Abstract

Funding

UN SDGs

Keywords

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