Structure-activity relationship in the mast cell degranulating capacity of neurotensin fragments

R. Sagi-Eisenberg*, Z. Ben-Neriah, I. Pecht, Sarah Terry, S. Blumberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The mast cell degranulating capacity of neurotensin and three of its fragments was examined. In Tyrode solution (137 mM NaCl, 2.7 mM KCl, 0.4 mM NaH2PO4, 1.4 mMCaCl2, 1 mM MgCl2, 10 mM Hepes, 5.6 mM glucose, pH 7.4), neither intact neurotensin nor its C-terminal tripeptide (Tyr-Ile-Leu) caused any release of histamine. Concentrations of neurotensin exceeding 10-4 M did cause histamine release but through lysis of the cells. The C-terminal hexa- and octapeptides of neurotensin (Arg-Arg-Pro-Tyr-Ile-Leu and Lys-Pro-Arg-Arg-Pro-Tyr-Ile-Leu, respectively) induced a non-cytolytic release of histamine with the latter peptide being more active (ED50 = 90 μM for the hexapeptide and 13 μM for the octapeptide). This release was not affected by the C-terminal tripeptide. It was found to be calcium-dependent and was inhibited by the anti-allergic drug, disodium cromoglycate. Phosphatidylserine did not enhance release of histamine and saturation of the immunoglobulin E (IgE) receptors did not inhibit it.

Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalNeuropharmacology
Volume22
Issue number2
DOIs
StatePublished - Feb 1983
Externally publishedYes

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