Striatal matrix neurons of the rat differentiate in culture from dissociated fetal progenitor cells isolated by buoyant density centrifugation

Amir Dori, Dragen Maric, Irina Maric, Rafik Masalha, Jeffrey L. Barker, William F. Silverman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

The adult striatum is composed of interlacing compartments known as patches (striosomes) and matrix, which differ with respect to a host of architectonic, biochemical and developmental parameters. We have exploited the 2-phase development of the striatum, employing buoyant-density fractionation to separate proliferating/undifferentiated neural precursors from the differentiated neurons of the E19 striatum. Primary cell cultures were established for the collected fractions, and immunohistochemistry for maturational and compartment-specific markers performed. The results indicate that the least buoyant, striatal precursors concentrate principally in the low buoyancy fraction of the gradient, and in culture express known matrix phenotype markers in an appropriate time frame. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)77-80
Number of pages4
JournalNeuroscience Letters
Volume282
Issue number1-2
DOIs
StatePublished - 17 Mar 2000
Externally publishedYes

Funding

FundersFunder number
Kreitman Foundation
WFS
National Institute of Neurological Disorders and StrokeZ01NS002019
Planning and Budgeting Committee of the Council for Higher Education of Israel

    Keywords

    • Basal ganglia
    • Development
    • Neural differentiation
    • Neural progenitor
    • Pattern formation

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