TY - JOUR
T1 - Striatal matrix neurons of the rat differentiate in culture from dissociated fetal progenitor cells isolated by buoyant density centrifugation
AU - Dori, Amir
AU - Maric, Dragen
AU - Maric, Irina
AU - Masalha, Rafik
AU - Barker, Jeffrey L.
AU - Silverman, William F.
N1 - Funding Information:
The antiserum for DARPP-32 was generously provided by Dr. H.C. Hemmings Jr. Thanks also to Ann Schaffner for her assistance with primary cultures and the Percoll fractionation technique. We are grateful for the technical assistance of Mrs. Valaria Frishman. This work was supported by the Council for Higher Education in Israel (WFS) and the Kreitman Foundation (AD).
PY - 2000/3/17
Y1 - 2000/3/17
N2 - The adult striatum is composed of interlacing compartments known as patches (striosomes) and matrix, which differ with respect to a host of architectonic, biochemical and developmental parameters. We have exploited the 2-phase development of the striatum, employing buoyant-density fractionation to separate proliferating/undifferentiated neural precursors from the differentiated neurons of the E19 striatum. Primary cell cultures were established for the collected fractions, and immunohistochemistry for maturational and compartment-specific markers performed. The results indicate that the least buoyant, striatal precursors concentrate principally in the low buoyancy fraction of the gradient, and in culture express known matrix phenotype markers in an appropriate time frame. Copyright (C) 2000 Elsevier Science Ireland Ltd.
AB - The adult striatum is composed of interlacing compartments known as patches (striosomes) and matrix, which differ with respect to a host of architectonic, biochemical and developmental parameters. We have exploited the 2-phase development of the striatum, employing buoyant-density fractionation to separate proliferating/undifferentiated neural precursors from the differentiated neurons of the E19 striatum. Primary cell cultures were established for the collected fractions, and immunohistochemistry for maturational and compartment-specific markers performed. The results indicate that the least buoyant, striatal precursors concentrate principally in the low buoyancy fraction of the gradient, and in culture express known matrix phenotype markers in an appropriate time frame. Copyright (C) 2000 Elsevier Science Ireland Ltd.
KW - Basal ganglia
KW - Development
KW - Neural differentiation
KW - Neural progenitor
KW - Pattern formation
UR - http://www.scopus.com/inward/record.url?scp=0034677859&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(00)00888-0
DO - 10.1016/S0304-3940(00)00888-0
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C2 - 10713400
AN - SCOPUS:0034677859
SN - 0304-3940
VL - 282
SP - 77
EP - 80
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1-2
ER -