Storage of glycoprotein in NCTR-Balb/C mouse - Lectin histochemistry, and biochemical studies

Hana Weintraub, Joseph Alroy*, Rita DeGasperi, Vibha Goyal, Ehud Skutelsky, Peter G. Pentchev, Christopher D. Warren

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


A strain of Balb/C mice carrying a lysosomal storage disorder exhibits metabolic and phenotypic abnormalities similar to patients with sphingomyelin-cholesterol lipidoses type II (i.e., Niemann-Pick C and D). Their foamy cells, which belong to the reticuloendothelial system, stained intensely by periodate-Schiff (PAS) reagent and were resistant to predigestion with diastase. To identify the chemical nature of the PAS-positive storage material, we applied lectin histochemistry and biochemical methods. Paraffin embedded sections, and delipidated frozen tissue sections, were treated with biotinylated lectins and localized with avidin-biotin-peroxidase complex. Araldite-embedded semithin sections were incubated with biotinylated lectins followed by avidin-gold and were enhanced with silver. By both histochemical methods the affected foamy cells stained positively as follows:Concanavalia ensiformis agglutinin, Datura stramonium agglutinin, Griffonia simplicifolia- I, Lens culinaris agglutinin, peanut agglutinin, Ricinus communis agglutinin-I, wheat germ agglutinin (WGA), and succinylated-WGA. Biochemical analysis of liver extracts complemented the histochemical data and demonstrated accumulation of glycoproteins containing polylactosaminoglycans in affected mice. Our findings indicate that the storage material in NCTR-Balb/C mice is heterogeneous. The lipids that are extracted by organic solvents during the histologic preparations mask the occurrence of polylactosaminoglycan containing glycoproteins in native frozen sections.

Original languageEnglish
Pages (from-to)347-352
Number of pages6
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Issue number1
StatePublished - Dec 1992


Dive into the research topics of 'Storage of glycoprotein in NCTR-Balb/C mouse - Lectin histochemistry, and biochemical studies'. Together they form a unique fingerprint.

Cite this