Stimulation of prostaglandin e and testosterone production in rat interstitial cells by a gonadotropin releasing hormone agonist

Jacob Molcho, Yona Eli, Haim Zakut, Zvi Naor

Research output: Contribution to journalArticlepeer-review

Abstract

The early direct effects of a GnRH agonist analog [D-Ala6]des-Gly10-GnRH N ethylamide (GnRHa) on rat testicular interstitial cells include increased production of prostaglandin E (PGE) and testosterone (T) at 3 h (ED50 values of 0.5 and 0.75 nM, respectively). On the other hand, LH action on testicular function, which is mediated by increased cAMP, involves an increase in T production at 30 min followed by increased PGE formation at 3 h. GnRHa at concentrations of 10-12-10-8 M had no effect on basal or LH-stimulated cAMP production during a 4-h incubation test. The stimulatory effect of GnRHa on PGE, but not on T production, was abolished by the prostaglandin synthesis inhibitor indomethacin (1.5 µnm). We conclude that cAMP does not play a role in mediating the direct testicular effects of GnRH on PGE and T production; that PGE is not involved in mediating GnRH-induced T production; and, finally, that increased PGE and T production might be involved in mediating the direct inhibitory and stimulatory testicular effects of GnRH and its agonist analogs.

Original languageEnglish
Pages (from-to)2382-2387
Number of pages6
JournalEndocrinology
Volume114
Issue number6
DOIs
StatePublished - 1984
Externally publishedYes

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