Stimulation of polymorphonuclear leukocytes by dicyclohexylcarbodiimide: calcium homeostasis

A. Aviram; I. Aviram

doi:10.1016/0143-4160(89)90051-1

Stimulation of polymorphonuclear leukocytes by dicyclohexylcarbodiimide: calcium homeostasis

A. Aviram, I. Aviram

Biochemistry Molecular Biology

Research output: Contribution to journal › Article › peer-review

Abstract

The involvement of calcium in N,N′-dicyclohexylcarbodiimide (DCCD)-mediated stimulation of guinea pig neutrophils was investigated. Exposure to DCCD resulted in a fast though moderate elevation of cytosolic calcium concentration. Exchange experiments indicated that DCCD enhanced ⁴⁵Ca²⁺ efflux without affecting uptake of the radioisotope from the medium. Plasma membranes isolated from DCCD-stimulated cells failed to support ATP-dependent ⁴⁵Ca²⁺ uptake indicating inhibition of their Ca-ATPase. The finding that the enhanced efflux of ⁴⁵Ca²⁺ depended on the presence of Na⁺ ions in the medium implicated a Na⁺ Ca²⁺ exchanger in efflux of the ion observed in DCCD-stimulated neutrophils. This is the first indication for the participation of this carrier in calcium homeostasis in stimulated neutrophils. Experiments carried out with ¹⁴C-DCCD indicated covaiont binding of the reagent to 20 and 150 Kd membrane proteins.

Original language	English
Pages (from-to)	115-124
Number of pages	10
Journal	Cell Calcium
Volume	10
Issue number	2
DOIs	https://doi.org/10.1016/0143-4160(89)90051-1
State	Published - 1989

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title = "Stimulation of polymorphonuclear leukocytes by dicyclohexylcarbodiimide: calcium homeostasis",

abstract = "The involvement of calcium in N,N′-dicyclohexylcarbodiimide (DCCD)-mediated stimulation of guinea pig neutrophils was investigated. Exposure to DCCD resulted in a fast though moderate elevation of cytosolic calcium concentration. Exchange experiments indicated that DCCD enhanced 45Ca2+ efflux without affecting uptake of the radioisotope from the medium. Plasma membranes isolated from DCCD-stimulated cells failed to support ATP-dependent 45Ca2+ uptake indicating inhibition of their Ca-ATPase. The finding that the enhanced efflux of 45Ca2+ depended on the presence of Na+ ions in the medium implicated a Na+ Ca2+ exchanger in efflux of the ion observed in DCCD-stimulated neutrophils. This is the first indication for the participation of this carrier in calcium homeostasis in stimulated neutrophils. Experiments carried out with 14C-DCCD indicated covaiont binding of the reagent to 20 and 150 Kd membrane proteins.",

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T1 - Stimulation of polymorphonuclear leukocytes by dicyclohexylcarbodiimide

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AU - Aviram, A.

AU - Aviram, I.

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N2 - The involvement of calcium in N,N′-dicyclohexylcarbodiimide (DCCD)-mediated stimulation of guinea pig neutrophils was investigated. Exposure to DCCD resulted in a fast though moderate elevation of cytosolic calcium concentration. Exchange experiments indicated that DCCD enhanced 45Ca2+ efflux without affecting uptake of the radioisotope from the medium. Plasma membranes isolated from DCCD-stimulated cells failed to support ATP-dependent 45Ca2+ uptake indicating inhibition of their Ca-ATPase. The finding that the enhanced efflux of 45Ca2+ depended on the presence of Na+ ions in the medium implicated a Na+ Ca2+ exchanger in efflux of the ion observed in DCCD-stimulated neutrophils. This is the first indication for the participation of this carrier in calcium homeostasis in stimulated neutrophils. Experiments carried out with 14C-DCCD indicated covaiont binding of the reagent to 20 and 150 Kd membrane proteins.

AB - The involvement of calcium in N,N′-dicyclohexylcarbodiimide (DCCD)-mediated stimulation of guinea pig neutrophils was investigated. Exposure to DCCD resulted in a fast though moderate elevation of cytosolic calcium concentration. Exchange experiments indicated that DCCD enhanced 45Ca2+ efflux without affecting uptake of the radioisotope from the medium. Plasma membranes isolated from DCCD-stimulated cells failed to support ATP-dependent 45Ca2+ uptake indicating inhibition of their Ca-ATPase. The finding that the enhanced efflux of 45Ca2+ depended on the presence of Na+ ions in the medium implicated a Na+ Ca2+ exchanger in efflux of the ion observed in DCCD-stimulated neutrophils. This is the first indication for the participation of this carrier in calcium homeostasis in stimulated neutrophils. Experiments carried out with 14C-DCCD indicated covaiont binding of the reagent to 20 and 150 Kd membrane proteins.

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Stimulation of polymorphonuclear leukocytes by dicyclohexylcarbodiimide: calcium homeostasis

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