Stimulation of polymorphonuclear leukocytes by dicyclohexylcarbodiimide: calcium homeostasis

A. Aviram, I. Aviram

Research output: Contribution to journalArticlepeer-review

Abstract

The involvement of calcium in N,N′-dicyclohexylcarbodiimide (DCCD)-mediated stimulation of guinea pig neutrophils was investigated. Exposure to DCCD resulted in a fast though moderate elevation of cytosolic calcium concentration. Exchange experiments indicated that DCCD enhanced 45Ca2+ efflux without affecting uptake of the radioisotope from the medium. Plasma membranes isolated from DCCD-stimulated cells failed to support ATP-dependent 45Ca2+ uptake indicating inhibition of their Ca-ATPase. The finding that the enhanced efflux of 45Ca2+ depended on the presence of Na+ ions in the medium implicated a Na+ Ca2+ exchanger in efflux of the ion observed in DCCD-stimulated neutrophils. This is the first indication for the participation of this carrier in calcium homeostasis in stimulated neutrophils. Experiments carried out with 14C-DCCD indicated covaiont binding of the reagent to 20 and 150 Kd membrane proteins.

Original languageEnglish
Pages (from-to)115-124
Number of pages10
JournalCell Calcium
Volume10
Issue number2
DOIs
StatePublished - 1989

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