Subcutaneous implantation of demineralized bone matrix in rats results in endochondral bone formation and bone-marrow development. The cascade of events leading to this process occurs following the accumulation of mesenchymal cells with colony forming cell fibroblasts (CFC-F) potential in the implanted area, a process which commences already 72 h post implantation. It is demonstrated herein that CFC-F from various stages of ossicle development (days, 3, 7, 10, 14 or 18) stimulate hemopoiesis to the same extent as judged by the number of granuloid-macrophage-progenitors (CFU-GM), developed as hemopoietic colonies, and by the ratio of granuloid to macrophagic colonies. High concentrations of CFC-F, however, tend to diminish the stimulatory capacity. Prostaglandin E, CFU-GM, CFC-F, ossicle, growth factors, microenvironment, hemopoiesis, development.
- 9 microenvironment
- growth factors