Stimulation of human monocyte oxidative burst and related cytotoxicity by tumor‐promoting and non‐tumorpromoting diterpene esters, indole alkaloids and polyacetate‐type agents

Yona Keisari*, Ita Geva, Eliezer Flescher, Hedva Goldin, Gad Lavie

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Human peripheral blood monocytes were cultured and exposed to plant diterpens, indole alkaloids and polyacetates with various degrees of tumor‐promoting activity. The effect of the above‐mentioned encounter on monocyte function was examined, as expressed by H2O2 production and lysis of dog erythrocytes by the cells, and the inhibition of 3H‐PDBu binding to the monocytes by the various test agents. The most effective reagents in both activation of monocyte function and inhibition of 3H‐PDBu binding were 12‐0‐tetradecanoyl‐phorbol 13‐acetate (TPA), phorbol 12, 13 dibutyrate, teleocidin, and aplysiatoxin which are known to be strong tumor promoters. Strong stimulation of monocyte function was also exerted by the weak tumor promoters, phorbol 12‐retinoate 13‐acetate, mezerein and debromoaplysiatoxin. Non‐tumor‐promoting phorbol diterpens such as phorbol 12, 13‐diacetate, phorbol 12‐myristate, phorbol 13‐acetate and 4‐alpha TPA were 1,000 times less effective than TPA in monocyte stimulation and inhibition of 3H‐PDBu binding. These results indicate that stimulation of human monocyte H2O2 production and related cytotoxicity might discriminate effectively between tumor‐promoting and non‐tumor‐promoting reagents.

Original languageEnglish
Pages (from-to)467-472
Number of pages6
JournalInternational Journal of Cancer
Volume36
Issue number4
DOIs
StatePublished - 15 Oct 1985

Funding

FundersFunder number
National Cancer InstituteR01CA012623

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