Stimulation of 42/44kDa mitogen-activated protein kinases by arginine vasopressin in rat cardiomyocytes

Orit Aharonovitz, Sharon Aboulafia-Etzion, Jonathan Leor, Battler Alexander Battler, Yosef Granot*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Vasoconstrictors, such as angiotensin II (Ang II), are involved in the regulatory mechanisms of post myocardial infarction (MI) hypertrophy. Arginine vasopressin (AVP), may be another vasoconstrictor that influences the mechanisms that lead to post MI hypertrophy. In these studies we investigated the possible activation of the 42/44 kDa mitogen-activated protein kinases (MAPKs), also referred as extracellular signal regulated kinases (ERKs), in cultured cardiomyocytes. Treatment of rat cardiomyocytes with AVP, Ang II and phorbol 12-myristate 13-acetate (PMA) increases the activation of ERKs. The activity of the 32/44 kDa MAPKs was tested using the phosphorylation of: (1) EGF receptor peptide (EGFR-P); (2) myelin basic protein (MBP) immobilized in poly acrylamide gels; and (3) T183 and Y185 residues of these proteins. The activity of the MAPKs, induced by AVP or PMA was inhibited by downregulation of protein kinase C (PKC), by the tyrosine kinase inhibitor genistein and by MAPK kinase (MEK) inhibitor, PD98059. In addition, the AVP-induced stimulation of MAPKs was shown to be mediated through a V, receptor. We suggest that AVP activates the 42/44 kDa MAPKs through a signal transduction pathway that involves stimulation of AVP-V1 receptor, tyrosine kinase, PKC and MEK. These results suggest that AVP may be involved in ERKs dependent regulatory functions of cardiomyocytes growth.

Original languageEnglish
Pages (from-to)105-111
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Cell Research
Volume1401
Issue number1
DOIs
StatePublished - 2 Jan 1998
Externally publishedYes

Funding

FundersFunder number
Gila and Arieh Keshet heart disease research fund

    Keywords

    • Arginine vasopressin
    • Cardiomyocyte
    • Extracellular signal regulated kinase
    • Mitogen-activated protein kinase
    • Protein kinase C
    • Tyrosine kinase

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