TY - JOUR
T1 - Sterilization of MacConkey agar and CLED medium by γ-radiation
AU - Bogokowsky, B.
AU - Eisenberg, E.
AU - Altmann, G.
PY - 1983/10
Y1 - 1983/10
N2 - MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by γ-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization by adding sodium thioglycollate as a radioprotectant, and by increasing their indicator content. The media performed well when tested with different Enterobacteria and other micro-organisms. Growth and change of indicator reaction were equal in irradiated and autoclaved culture media. Culture media were also evaluated after storage for one month at room temperature and at 4°C and compared well with freshly autoclaved media.
AB - MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by γ-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization by adding sodium thioglycollate as a radioprotectant, and by increasing their indicator content. The media performed well when tested with different Enterobacteria and other micro-organisms. Growth and change of indicator reaction were equal in irradiated and autoclaved culture media. Culture media were also evaluated after storage for one month at room temperature and at 4°C and compared well with freshly autoclaved media.
UR - http://www.scopus.com/inward/record.url?scp=0020840828&partnerID=8YFLogxK
U2 - 10.1016/0020-708X(83)90044-3
DO - 10.1016/0020-708X(83)90044-3
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
AN - SCOPUS:0020840828
SN - 0020-708X
VL - 34
SP - 1441
EP - 1443
JO - Applied Radiation and Isotopes
JF - Applied Radiation and Isotopes
IS - 10
ER -