Sterilization of MacConkey agar and CLED medium by γ-radiation

B. Bogokowsky, E. Eisenberg, G. Altmann

Research output: Contribution to journalArticlepeer-review

Abstract

MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by γ-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization by adding sodium thioglycollate as a radioprotectant, and by increasing their indicator content. The media performed well when tested with different Enterobacteria and other micro-organisms. Growth and change of indicator reaction were equal in irradiated and autoclaved culture media. Culture media were also evaluated after storage for one month at room temperature and at 4°C and compared well with freshly autoclaved media.

Original languageEnglish
Pages (from-to)1441-1443
Number of pages3
JournalApplied Radiation and Isotopes
Volume34
Issue number10
DOIs
StatePublished - Oct 1983
Externally publishedYes

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