Antisera against propranolol were produced in rabbits immunized with propranolol conjugated to bovine serum albumin. The antiserum against dl propranolol recognized both d and l propranolol to the same degree. However, antiserum against l propranolol was able to discriminate the l propranolol selectively. The antisera were used to develop radioimmunoassays for dl propranolol and l propranolol. The assay can detect as little as 10 pg of propranolol. Metabolites of propranolol do not interfere with the assay unless concentrations are very high. Serum and heart levels of l propranolol and the d isomer were determined in the rat after i.v. injection (1 mg/kg) of dl propranolol. l Propanolol declines rapidly in the blood after the injection. Concomitantly, there is a rapid accumulation of l propranolol by the heart. The d form of propranolol remains in the blood and is metabolized rapidly as reflected by a shorter half life (23.8 minutes) than the one found for l propranolol (52.0 minutes).
|Number of pages||7|
|Journal||Journal of Pharmacology and Experimental Therapeutics|
|State||Published - 1976|