TY - JOUR
T1 - Stem cells of the lamina propria of human oral mucosa and gingiva develop into mineralized tissues in vivo
AU - Treves-Manusevitz, Sandra
AU - Hoz, Lia
AU - Rachima, Heled
AU - Montoya, Gonzalo
AU - Tzur, Ephraim
AU - Vardimon, Alexander
AU - Narayanan, A. Sampath
AU - Amar, Salomon
AU - Arzate, Higinio
AU - Pitaru, Sandu
PY - 2013/1
Y1 - 2013/1
N2 - Aim:s To characterize the mineralized tissue formed constitutively in the supracalvarial region of scid mice by a primitive stem cell population (hOMSC) derived from the lamina propria of the human oral mucosa and gingiva. Material and Methods Fibrin-hOMSC constructs were cultured for 14 days at which time point they were analysed for the expression of osteoblastic/cementoblastic markers and implanted between the skin and calvaria bones into scid mice. After 8 weeks, the animals were sacrificed and the implantation sites analysed. Results: Two-week-old cultures of fibrin-hOMSC constructs expressed osteogenic/cementogenic markers at the gene level. Macroscopic and radiographic examinations revealed mineralized masses at the implantation sites of fibrin-hOMSC constructs. Histology, histochemistry and immunofluorescence showed mineralized masses consisting of avascular cellular and acellular matrices that stained positively for collagen, Ca, cementum attachment protein, cementum protein 1, bone sialoprotein, alkaline phosphatase, osteocalcin, amelogenin and ameloblastin. Positive anti-human nuclear antigen indicated the human origin of the cells. Atomic force microscopy depicted long prismatic structures organized in lamellar aggregates. Conclusion:s Within the limitation of this study, the results indicate for the first time that fibrin-hOMSC constructs are endowed with the constitutive capacity to develop into mineralized tissues that exhibit certain similarities to cementum and bone.
AB - Aim:s To characterize the mineralized tissue formed constitutively in the supracalvarial region of scid mice by a primitive stem cell population (hOMSC) derived from the lamina propria of the human oral mucosa and gingiva. Material and Methods Fibrin-hOMSC constructs were cultured for 14 days at which time point they were analysed for the expression of osteoblastic/cementoblastic markers and implanted between the skin and calvaria bones into scid mice. After 8 weeks, the animals were sacrificed and the implantation sites analysed. Results: Two-week-old cultures of fibrin-hOMSC constructs expressed osteogenic/cementogenic markers at the gene level. Macroscopic and radiographic examinations revealed mineralized masses at the implantation sites of fibrin-hOMSC constructs. Histology, histochemistry and immunofluorescence showed mineralized masses consisting of avascular cellular and acellular matrices that stained positively for collagen, Ca, cementum attachment protein, cementum protein 1, bone sialoprotein, alkaline phosphatase, osteocalcin, amelogenin and ameloblastin. Positive anti-human nuclear antigen indicated the human origin of the cells. Atomic force microscopy depicted long prismatic structures organized in lamellar aggregates. Conclusion:s Within the limitation of this study, the results indicate for the first time that fibrin-hOMSC constructs are endowed with the constitutive capacity to develop into mineralized tissues that exhibit certain similarities to cementum and bone.
KW - bone
KW - cementum
KW - gingiva
KW - oral mucosa
KW - regeneration
KW - stem cells
UR - http://www.scopus.com/inward/record.url?scp=84870859513&partnerID=8YFLogxK
U2 - 10.1111/jcpe.12016
DO - 10.1111/jcpe.12016
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C2 - 23137193
AN - SCOPUS:84870859513
SN - 0303-6979
VL - 40
SP - 73
EP - 81
JO - Journal of Clinical Periodontology
JF - Journal of Clinical Periodontology
IS - 1
ER -