Standalone cohesin as a molecular shuttle in cellulosome assembly

Milana Voronov-Goldman, Oren Yaniv, Ozgur Gul, Hagar Yoffe, Orly Salama-Alber, Michal Slutzki, Maly Levy-Assaraf, Sadanari Jindou, Linda J.W. Shimon, Ilya Borovok, Edward A. Bayer, Raphael Lamed, Felix Frolow

Research output: Contribution to journalArticlepeer-review

Abstract

The cellulolytic bacterium Ruminococcus flavefaciens of the herbivore rumen produces an elaborate cellulosome system, anchored to the bacterial cell wall via the covalently bound scaffoldin ScaE. Dockerin-bearing scaffoldins also bind to an autonomous cohesin of unknown function, called cohesin G (CohG). Here, we demonstrate that CohG binds to the scaffoldin-borne dockerin in opposite orientation on a distinct site, relative to that of ScaE. Based on these structural data, we propose that the complexed dockerin is still available to bind ScaE on the cell surface. CohG may thus serve as a molecular shuttle for delivery of scaffoldins to the bacterial cell surface.

Original languageEnglish
Pages (from-to)1569-1576
Number of pages8
JournalFEBS Letters
Volume589
Issue number14
DOIs
StatePublished - 3 Jun 2015

Keywords

  • Cellulose degradation
  • Cohesin
  • Cohesin-dockerin complex
  • Dockerin
  • Rumen bacteria
  • Ruminococcus flavefaciens
  • Type III

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