Standalone cohesin as a molecular shuttle in cellulosome assembly

Milana Voronov-Goldman, Oren Yaniv, Ozgur Gul, Hagar Yoffe, Orly Salama-Alber, Michal Slutzki, Maly Levy-Assaraf, Sadanari Jindou, Linda J.W. Shimon, Ilya Borovok, Edward A. Bayer*, Raphael Lamed, Felix Frolow

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

The cellulolytic bacterium Ruminococcus flavefaciens of the herbivore rumen produces an elaborate cellulosome system, anchored to the bacterial cell wall via the covalently bound scaffoldin ScaE. Dockerin-bearing scaffoldins also bind to an autonomous cohesin of unknown function, called cohesin G (CohG). Here, we demonstrate that CohG binds to the scaffoldin-borne dockerin in opposite orientation on a distinct site, relative to that of ScaE. Based on these structural data, we propose that the complexed dockerin is still available to bind ScaE on the cell surface. CohG may thus serve as a molecular shuttle for delivery of scaffoldins to the bacterial cell surface.

Original languageEnglish
Pages (from-to)1569-1576
Number of pages8
JournalFEBS Letters
Volume589
Issue number14
DOIs
StatePublished - 3 Jun 2015

Funding

FundersFunder number
Helmsley Foundation
Israel Ministry of Science604530
Israel Science Foundation – Israel
Israel Strategic Alternative Energy Foundation
Israeli Center of Research Excellence
Sidney E. Frank Foundation
Weizmann Institute of Science Alternative Energy Research Initiative
Archival Education and Research Institute
Japan Society for the Promotion of Science26850224
United States-Israel Binational Science Foundation
Israel Science Foundation1349/13, 24/11, 445293/08
Israeli Centers for Research Excellence152/11

    Keywords

    • Cellulose degradation
    • Cohesin
    • Cohesin-dockerin complex
    • Dockerin
    • Rumen bacteria
    • Ruminococcus flavefaciens
    • Type III

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