Stability of the pBR322 plasmid as affected by the promoter region of the tetracycline-resistance gene

Mikhail N. Kolot, Mikhail V. Kashlev, Alexandr I. Gragerov, Inessa A. Khmel

Research output: Contribution to journalArticlepeer-review

Abstract

A region affecting the pBR322 plasmid maintenance has been located within the region of the TcR gene promoter. On the basis of stability analysis of pBR322 derivatives comprising the modified region of the TcR gene, we deduced that it is the nucleotide sequence localized in the region of the HindIII site that causes destabilization of the plasmid and not the TcR gene product or active transcription of this region. The destabilizing effect is manifested both in cis and in trans.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalGene
Volume75
Issue number2
DOIs
StatePublished - 20 Feb 1989
Externally publishedYes

Keywords

  • Recombinant DNA
  • deletions
  • pACYC184

Fingerprint

Dive into the research topics of 'Stability of the pBR322 plasmid as affected by the promoter region of the tetracycline-resistance gene'. Together they form a unique fingerprint.

Cite this