Spermatogenesis in the golden hamster during the first spermatogenic wave: A flow cytometric analysis

R. Golan*, R. Weissenberg, Y. Oschry, L. Shochat, L. M. Lewin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

In the present study propidium iodide was used as a fluorescent dye to stain DNA of cells of hamster testicular origin and fluorescent intensities were analyzed by flow cytometry. We used hamster testicular cells from the first spermatogenic wave to observe the consecutive appearance of the different types of cells during puberty. At 12 days postpartum (dpp) diploid cells (including spermatogonia) predominated and some tetraploid cells were also present. Tetraploid spermatocytes increased dramatically by 21 dpp. The first haploid cells appeared at 21 dpp but substantial numbers were first present at 23 dpp. Immature haploid cells predominated at 32 dpp. Elongating condensing spermatids appeared at 34 dpp and spermatozoa began to leave the testis to enter the epididymidis at 36-38 dpp marking the end of the first round of spermatogenesis. Using acridine orange staining flow cytometry, chromatin condensation was followed by measuring fluorescence decrease from early round spermatids to spermatozoa obtained from the initial segment and from the cauda epididymides. The major portion of sperm chromatin condensation (8890%) in the hamster occurred in the testis and only 10-12% occurred during epididymal sperm maturation. Spermatozoa in the initial segment of the epididymidis of the hamster contained a small amount of RNA that was no longer present in sperm of the cauda epididymidis, indicating that RNA was lost during epididymal sperm maturation in this species.

Original languageEnglish
Pages (from-to)205-211
Number of pages7
JournalMolecular Reproduction and Development
Volume55
Issue number2
DOIs
StatePublished - 2000

Keywords

  • First spermatogenic wave
  • Flow cytometry
  • Golden hamster
  • Puberty
  • Spermatogenesis

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