Sperm inspection for in vitro fertilization via self-assembled microdroplet formation and quantitativephase microscopy

Yuval Atzitz, Matan Dudaie, Itay Barnea, Natan T. Shaked*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

We present a new method for the selection of individual sperm cells using a microfluidic de-vice that automatically traps each cell in a separate microdroplet that then individually self-assembles with other microdroplets, permitting the controlled measurement of the cells using quantitative phase microscopy. Following cell trapping and droplet formation, we utilize quantitative phase microscopy integrated with bright-field imaging for individual sperm morphology and motility inspection. We then perform individual sperm selection using a single-cell micromanipulator, which is enhanced by the microdroplet-trapping procedure described above. This method can improve sperm selection for intracytoplasmic sperm injection, a common type of in vitro fertilization procedure.

Original languageEnglish
Article number3317
JournalCells
Volume10
Issue number12
DOIs
StatePublished - Dec 2021

Keywords

  • Digital holographic microscopy
  • Quantitative phase imaging
  • Sperm cells

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