Spectroscopic studies on the tyrosine residues of canavalin

Rimona Margalit*, Nurith Shaklai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Canavalin is a tetramer with 6 tyrosines per subunit. In the work presented here, we have classified these tyrosines by their spectrophotometric and fluorometric pH titration and their ability to be quenched I-. Of the 6 residues, 2 were found to be exposed to the solvent. One (pK = 10.2) contributes 28% of the total fluorescence intensity; the second has a pK of 11.50, and a lower quantum yield, contributing only 16% of the total intensity. The remaining 4 residues (pK = 12.5, contributing 54% of fluorescence intensity) are buried; their titration is irreversible, requiring protein denaturation.

Original languageEnglish
Pages (from-to)272-280
Number of pages9
JournalBBA - Protein Structure
Volume535
Issue number2
DOIs
StatePublished - 21 Aug 1978

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