Spectral morphometric characterization of B-CLL cells versus normal small lymphocytes

Zvi Malik*, Chana Rothmann, Tova Cycowitz, Zwi J. Cycowitz, Amos M. Cohen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Spectral morphometric characterization of typical chronic lymphocytic leukemia (B-CLL) cells vs normal small lymphocytes stained by May-Grunwald- Giemsa was carried out by multipixel spectral imaging. The light intensity (450-850 nm of 104 pixels) from nuclear domains of each stained cell was recorded and represented as light transmittance spectra and optical density. Transmitted light spectra of two nuclear domains were determined, one with low-intensity light transmittance (LIT) and the other with high-intensity light transmittance (HIT). A spectral library was constructed using the four transmitted light spectra representing the HIT and LIT domains of the normal human lymphocytes and the LIT and HIT domains of the CLL cells. The spectral library served to scan CLL lymphocytes from 10 cases of CLL and the lymphocytes of 10 healthy individuals. Each spectrally similar domain in the nuclei of the lymphocytes was assigned an arbitrary color. The morphometric analysis of the spectrally classified nuclei showed specific spectral patterns for B-CLL in 92% of the cells. The specific spectral characteristics of each of the two cell populations were also observed by their optical density light absorbance spectra. We propose that spectral morphometric analysis may serve as an additional diagnostic tool for detection of CLL lymphocytes in a hematological specimen.

Original languageEnglish
Pages (from-to)1113-1118
Number of pages6
JournalJournal of Histochemistry and Cytochemistry
Volume46
Issue number10
DOIs
StatePublished - Oct 1998
Externally publishedYes

Keywords

  • Absorbance image
  • CLL
  • Classified image
  • Multipixel spectroscopy
  • Spectral similarity mapping

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