TY - JOUR
T1 - Specific stimulation of RNA synthesis by methionine in several strains of Escherichia coli
AU - Ron, Eliora Z.
AU - Davis, Bernard D.
N1 - Funding Information:
These studies are to be submitted to Harvard University by one of us (E. Z. R.) in partial fulfillment of the requirements for the degree of Doctor of Philosophy. This work was supported by grant GB1307 of the National Science Foundation and by training grant GM177-07 of the Public Health Service.
PY - 1966/10/28
Y1 - 1966/10/28
N2 - The addition of methionine to a growing culture of certain strains of Escherichia coli causes a preferential stimulation of RNA synthesis. In the new steady state the RNA/protein ratio is increased 25% or more, with little change in growth rate. The excess RNA is found largely in ribosomes and appears to be normally methylated. The rate of restoration of the normal RNA/protein ratio after removal of methionine suggests that the extra ribosomes, though not contributing to the rate of protein synthesis, are functional. Possible mechanisms to explain this variation in the control of RNA synthesis are discussed.
AB - The addition of methionine to a growing culture of certain strains of Escherichia coli causes a preferential stimulation of RNA synthesis. In the new steady state the RNA/protein ratio is increased 25% or more, with little change in growth rate. The excess RNA is found largely in ribosomes and appears to be normally methylated. The rate of restoration of the normal RNA/protein ratio after removal of methionine suggests that the extra ribosomes, though not contributing to the rate of protein synthesis, are functional. Possible mechanisms to explain this variation in the control of RNA synthesis are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0014030654&partnerID=8YFLogxK
U2 - 10.1016/0022-2836(66)90076-3
DO - 10.1016/0022-2836(66)90076-3
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AN - SCOPUS:0014030654
SN - 0022-2836
VL - 21
SP - 13
EP - 27
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 1
ER -